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  • KD/KO Validated

ZMYND8 Polyclonal antibody

ZMYND8 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

IHC, IP, WB, ELISA and More (2)

Conjugate

Unconjugated

Cat No : 11633-1-AP

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Synonyms

CTCL associated antigen se14 3, KIAA1125, PRKCBP1, PRO2893, RACK7, ZMYND8



经过测试的应用

Positive WB detected inMCF-7 cells, HeLa cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ZMYND8 Ready-To-Use IHC Kit. ZMYND8 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

11633-1-AP targets ZMYND8 in WB, IP, IF, IHC, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications IHC, IP, WB, ELISA
Cited ApplicationsWB, IF, IHC, ChIP
Tested Reactivity human
Cited Reactivityhuman, mouse
Immunogen ZMYND8 fusion protein Ag2207 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger, MYND-type containing 8
Synonyms CTCL associated antigen se14 3, KIAA1125, PRKCBP1, PRO2893, RACK7, ZMYND8
Calculated Molecular Weight 1186 aa, 132 kDa
Observed Molecular Weight 150 kDa
GenBank Accession NumberBC030721
Gene Symbol ZMYND8
Gene ID (NCBI) 23613
RRIDAB_2241834
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9ULU4
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Regulation of neuronal gene expression is essential to nervous system development. REST (RE1-silencing transcription factor) regulates neuronal gene expression by interacting with a group of corepressor proteins including REST corepressors (RCOR). ZMYND8 bind to ROCR2 and can function as transcriptional repressors. It is co-expressed with RCOR2 in the nervous system and overexpression of ZMYND8 inhibits neural differentiation. [PMID:20331974]

实验方案

Product Specific Protocols
WB protocol for ZMYND8 antibody 11633-1-APDownload protocol
IHC protocol for ZMYND8 antibody 11633-1-APDownload protocol
IP protocol for ZMYND8 antibody 11633-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouse,human

Mol Cell

The ZMYND8-regulated mevalonate pathway endows YAP-high intestinal cancer with metabolic vulnerability.

Authors - Qiang Pan
  • KO Validated
humanWB

Mol Cancer

LncRNA TROJAN promotes proliferation and resistance to CDK4/6 inhibitor via CDK2 transcriptional activation in ER+ breast cancer.

Authors - Xi Jin
  • KD Validated
humanWB,IHC,IF

Sci Adv

The endogenous retrovirus-derived long noncoding RNA TROJAN promotes triple-negative breast cancer progression via ZMYND8 degradation.

Authors - Xi Jin
mouseChIP

J Tissue Eng

Mesenchymal stem cells overexpressing XIST induce macrophage M2 polarization and improve neural stem cell homeostatic microenvironment, alleviating spinal cord injury

Authors - Dan Zhu