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验证数据展示

  • WB analysis of PC-3 using 12847-1-AP (same clone as 12847-1-PBS)

    WB analysis of PC-3 using 12847-1-AP (same clone as 12847-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12847-1-AP (ZMPSTE24 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12847-1-PBS in a different storage buffer formulation.

  • IP experiment of PC-3 using 12847-1-AP (same clone as 12847-1-PBS)

    IP experiment of PC-3 using 12847-1-AP (same clone as 12847-1-PBS)

    IP result of anti-ZMPSTE24 (IP:12847-1-AP, 4ug; Detection:12847-1-AP 1:300) with PC-3 cells lysate 3200ug. This data was developed using the same antibody clone with 12847-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 12847-1-AP (same clone as 12847-1-PBS)

    IF Staining of PC-3 using 12847-1-AP (same clone as 12847-1-PBS)

    Immunofluorescent analysis of PC-3 cells using 12847-1-AP (ZMPSTE24 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12847-1-PBS in a different storage buffer formulation.

ZMPSTE24 Polyclonal antibody, PBS Only

ZMPSTE24 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 12847-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZMPSTE24 Ready-To-Use IHC Kit. ZMPSTE24 primary antibody included.

产品信息

12847-1-PBS targets ZMPSTE24 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3949

Product name: Recombinant human ZMPSTE24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 225-475 aa of BC037283

Sequence: TPLPEGKLKEEIEVMAKSIDFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEEYSVLNKDIQEDSGMEPRNEEEGNSEEIKAKVKNKKQGCKNEEVLAVLGHELGHWKLGHTVKNIIISQMNSFLCFFLFAVLIGRKELFAAFGFYDSQPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADAFAKKLGKAKDLYSALIKLNKDNLGFPVSDWLFSMWHYSHPPLLERLQALKTMKQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc metallopeptidase (STE24 homolog, S. cerevisiae)
别名
计算分子量 475 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC037283
基因名称 ZMPSTE24
Gene ID (NCBI) 10269
RRIDAB_2257430
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75844
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

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