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验证数据展示

  • WB analysis of PC-3 using 12847-1-AP

    WB analysis of PC-3 using 12847-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 12847-1-AP (ZMPSTE24 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 12847-1-AP

    IP experiment of PC-3 using 12847-1-AP

    IP result of anti-ZMPSTE24 (IP:12847-1-AP, 4ug; Detection:12847-1-AP 1:300) with PC-3 cells lysate 3200ug.

  • IF Staining of PC-3 using 12847-1-AP

    IF Staining of PC-3 using 12847-1-AP

    Immunofluorescent analysis of PC-3 cells using 12847-1-AP (ZMPSTE24 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

ZMPSTE24 Polyclonal antibody

ZMPSTE24 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 12847-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

Farnesylated proteins-converting enzyme 1, FACE-1, FACE1, FACE 1, EC:3.4.24.84

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inPC-3 cells
Positive IP detected inPC-3 cells
Positive IF/ICC detected inPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy ZMPSTE24 Ready-To-Use IHC Kit. ZMPSTE24 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

12847-1-AP targets ZMPSTE24 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag3949

Product name: Recombinant human ZMPSTE24 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 225-475 aa of BC037283

Sequence: TPLPEGKLKEEIEVMAKSIDFPLTKVYVVEGSKRSSHSNAYFYGFFKNKRIVLFDTLLEEYSVLNKDIQEDSGMEPRNEEEGNSEEIKAKVKNKKQGCKNEEVLAVLGHELGHWKLGHTVKNIIISQMNSFLCFFLFAVLIGRKELFAAFGFYDSQPTLIGLLIIFQFIFSPYNEVLSFCLTVLSRRFEFQADAFAKKLGKAKDLYSALIKLNKDNLGFPVSDWLFSMWHYSHPPLLERLQALKTMKQH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc metallopeptidase (STE24 homolog, S. cerevisiae)
别名 Farnesylated proteins-converting enzyme 1, FACE-1, FACE1, FACE 1, EC:3.4.24.84
计算分子量 475 aa, 55 kDa
观测分子量 55 kDa
GenBank蛋白编号BC037283
基因名称 ZMPSTE24
Gene ID (NCBI) 10269
RRIDAB_2257430
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDO75844
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZMPSTE24(CAAX prenyl protease 1 homolog) gene encodes a zinc metalloproteinase involved in the processing of farnesylated proteins(PubMed: 10373325). The significance of ZMPSTE24 in human disease stems from its role as an enzyme necessary for the correct processing and maturation of lamin A ,an intermediate filament component of the nuclear envelope (PubMed: 16297189).

实验方案

Product Specific Protocols
IF protocol for ZMPSTE24 antibody 12847-1-APDownload protocol
IP protocol for ZMPSTE24 antibody 12847-1-APDownload protocol
WB protocol for ZMPSTE24 antibody 12847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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