ZG16 Monoclonal antibody
ZG16 Monoclonal Antibody for IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, pig, rat and More (1)
Applications
IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
1A7B9
验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue, human colon tissue, pig colon tissue |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | mouse small intestine tissue |
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 594 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IHC | See 1 publications below |
产品信息
67389-1-Ig targets ZG16 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse, pig, rat samples.
| Tested Applications | IF-P, IHC, WB, ELISA |
| Cited Applications | IHC |
| Tested Reactivity | Human, mouse, pig, rat |
| Cited Reactivity | human |
| Immunogen | ZG16 fusion protein Ag11332 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | zymogen granule protein 16 homolog (rat) |
| Synonyms | FLJ43571, FLJ92276, hZG16, JCLN, JCLN1, Secretory lectin ZG16, ZG16, ZG16A, Zymogen granule protein 16 |
| Calculated Molecular Weight | 167 aa, 18 kDa |
| Observed Molecular Weight | 16 kDa |
| GenBank Accession Number | BC029149 |
| Gene Symbol | ZG16 |
| Gene ID (NCBI) | 653808 |
| RRID | AB_2882633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | O60844 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Zymogen granule protein 16 (ZG16) has a Jacalin-like lectin domain, which is mainly expressed by mucus-secreting cells, including goblet cells in the intestine. It's reported that ZG16 expression was significantly decreased in colorectal cancer compared to normal tissue. ZG16 gene expression and copy number variations (CNV) were associated with multiple molecular and clinicopathological features of CRC including MSI, MLH1 silencing and so on. It's found that ZG16 is negatively correlated with lymphatic invasive and distant metastasis. Besides, overexpression of ZG16 blocks PD-L1 expression in colorectal cancer, and promotes NK cells survival and proliferation. Very importantly, ZG16 suppresses colorectal tumor growth via the immune system. (PMID: 33360840, 21893569)
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZG16 antibody 67389-1-Ig | Download protocol |
| IHC protocol for ZG16 antibody 67389-1-Ig | Download protocol |
| IF protocol for ZG16 antibody 67389-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Elife Proteomic characteristics reveal the signatures and the risks of T1 colorectal cancer metastasis to lymph nodes |
