验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells |
| Positive IHC detected in | human colon cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse small intestine tissue |
| Positive IF-Fro detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
20350-1-AP targets SLC1A5/ASCT2 in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, Blocking assay applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, Blocking assay |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human, mouse, rat, pig, canine, goat |
| 免疫原 |
CatNo: Ag14182 Product name: Recombinant human RDRC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 436-541 aa of BC000062 Sequence: VLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPLPTEEGNPLLKHYRGPAGDATVASEKESVM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 1 (neutral amino acid transporter), member 5 |
| 别名 | SLC1A5, AAAT, ASCT2, ATB(0), ATBO |
| 计算分子量 | 541 aa, 57 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC000062 |
| 基因名称 | SLC1A5/ASCT2 |
| Gene ID (NCBI) | 6510 |
| RRID | AB_2878679 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15758 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for SLC1A5/ASCT2 antibody 20350-1-AP | Download protocol |
| IF protocol for SLC1A5/ASCT2 antibody 20350-1-AP | Download protocol |
| IHC protocol for SLC1A5/ASCT2 antibody 20350-1-AP | Download protocol |
| WB protocol for SLC1A5/ASCT2 antibody 20350-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Science Family single-cell atlases reveal pig pregnancy and fetal growth restriction critical cell types.
| ||
Cancer Res ASS1-mediated reductive carboxylation of cytosolic glutamine confers ferroptosis resistance in cancer cells | ||
ACS Nano Biomimetic Nanomedicine Targeting Orchestrated Metabolism Coupled with Regulatory Factors to Disrupt the Metabolic Plasticity of Breast Cancer | ||
ACS Nano Nutritional Glutamine-Modified Iron-Delivery System with Enhanced Endocytosis for Ferroptosis Therapy of Pancreatic Tumors |
