验证数据展示
经过测试的应用
| Positive IF-P detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | HEK-293 cells |
| Positive FC (Intra) detected in | HeLa cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-20350 targets ASCT2 in IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse samples.
| 经测试应用 | IF/ICC, IF-P, FC (Intra) Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag14182 Product name: Recombinant human RDRC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 436-541 aa of BC000062 Sequence: VLTLAIILEAVNLPVDHISLILAVDWLVDRSCTVLNVEGDALGAGLLQNYVDRTESRSTEPELIQVKSELPLDPLPLPTEEGNPLLKHYRGPAGDATVASEKESVM 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | solute carrier family 1 (neutral amino acid transporter), member 5 |
| 别名 | SLC1A5/ASCT2, SLC1A5, AAAT, ATB(0), ATBO |
| 计算分子量 | 541 aa, 57 kDa |
| 观测分子量 | 55-70 kDa |
| GenBank蛋白编号 | BC000062 |
| 基因名称 | SLC1A5/ASCT2 |
| Gene ID (NCBI) | 6510 |
| RRID | AB_3084033 |
| 偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
| 最大激发/发射波长 | 493 nm / 522 nm |
| 激发激光 | Blue laser (488 nm) |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q15758 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
SLC1A5 (Solute Carrier Family 1, member 5), also named as ASCT2, a major glutamine transporter belonging to the SLC1 family and localized in the plasma membrane of several body districts. Consistent with the functions exerted by glutamine, SLC1A5 is involved in uptake of essential amino acids, activation of mTORC1 and glutamine-dependent tumor cell survival and growth. SLC1A5 is highly expressed in various malignancies and plays a critical role in the transformation, growth and survival of cancer cells (PMID: 30234109). High SLC1A5 expression is associated with poor prognosis in clear-cell renal cell carcinoma (PMID: 26599282).
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 488 ASCT2 antibody CL488-20350 | Download protocol |
| IF protocol for CL Plus 488 ASCT2 antibody CL488-20350 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
