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验证数据展示

  • WB analysis using 25360-1-AP

    WB analysis using 25360-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 25360-1-AP (ZFYVE28 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 25360-1-AP

    IF Staining of HepG2 using 25360-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IF Staining of HeLa using 25360-1-AP

    IF Staining of HeLa using 25360-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZFYVE28 antibody (25360-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

ZFYVE28 Polyclonal antibody

ZFYVE28 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 25360-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, ELISA

别名

Zinc finger FYVE domain-containing protein 28, LST2, KIAA1643, hLst2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inMCF-7 cells
Positive IF/ICC detected inHepG2 cells, HeLa cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:3000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25360-1-AP targets ZFYVE28 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag19796

Product name: Recombinant human ZFYVE28 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 575-887 aa of BC137309

Sequence: EDVVERLREKCSPGGVIGASYAAGLAKASDRAPERQEEAPPPSEDASNGREPKAPTSDKCLPHTSGSQVDTASGLQGEAGVAGQQEPEARELHAGSPPAHEAPQALSGSSSSTAGSCSSDKMGPEAAPAATHAAPQATREKIRSRFHGSHDLIHRLFVCISGVADQLQTNYASDLRSILKTLFEVMATKPETDDKEKLRKVTQTLRSAALEDCALCQETLSSSELAAKTRDGDFEDPPEWVPDEACGFCTACKAPFTVIRRKHHCRSCGKIFCSRCSSHSAPLPRYGQVKPVRVCTHCYMFHVTPFYSDKAGL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger, FYVE domain containing 28
别名 Zinc finger FYVE domain-containing protein 28, LST2, KIAA1643, hLst2
计算分子量 887 aa, 96 kDa
观测分子量 96 kDa
GenBank蛋白编号BC137309
基因名称 ZFYVE28
Gene ID (NCBI) 57732
RRIDAB_3085790
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9HCC9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZFYVE28, also known as LST2, belongs to the lst-2 family. ZFYVE28 is a FYVE domain protein that has been shown to be a regulator of the epidermal growth factor receptor (EGFR) pathway. The non-ubiquitinylated form of ZFYVE28 co-localizes with EGFR in endosomes and promotes receptor degradation (PMID: 29549365). ZFYVE28 has 8 isoforms with the molecular mass of 19-33, 83-93, and 96 kDa.

实验方案

Product Specific Protocols
IF protocol for ZFYVE28 antibody 25360-1-APDownload protocol
WB protocol for ZFYVE28 antibody 25360-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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