验证数据展示
经过测试的应用
| Positive WB detected in | mouse thymus tissue, mouse testis tissue, mouse brain tissue, Jurkat cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
12680-1-AP targets Protrudin in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF, IP, CoIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, monkey |
| 免疫原 |
CatNo: Ag3369 Product name: Recombinant human ZFYVE27 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 217-411 aa of BC030621 Sequence: VEFFRVVSEYRASLQQRMNPKQEEHAFESPPPPDVGGKDGLMDSTPALTPTEDLTPGSVEEAEEAEPDEEFKDAIEETHLVVLEDDEGAPCPAEDELALQDNGFLSKNEVLRSKVSRLTERLRKRYPTNNFGNCTGCSATFSVLKKRRSCSNCGNSFCSRCCSFKVPKSSMGATAPEAQRETVFVCASCNQTLSK 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger, FYVE domain containing 27 |
| 别名 | ZFYVE27, Zinc finger FYVE domain-containing protein 27, SPG33, Spastic paraplegia 33 protein, RP11 459F3.2 |
| 计算分子量 | 411 aa, 46 kDa |
| 观测分子量 | 46-50 kDa |
| GenBank蛋白编号 | BC030621 |
| 基因名称 | ZFYVE27 |
| Gene ID (NCBI) | 118813 |
| RRID | AB_10640298 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5T4F4 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Protrudin, also named as ZFYVE27, is a 411 amino acid protein, which contains 1 FYVE-type zinc finger. Protrudin localizes in the recycling endosome membrane and functions as an upstream inhibitor of RAB11, regulating directional protein transport to the forming neurites. Protrudin is a key regulator of vesicular transport during neurite extension and is involved in neuronal intracellular trafficking in the corticospinal tract.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for Protrudin antibody 12680-1-AP | Download protocol |
| WB protocol for Protrudin antibody 12680-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun PDZD8 interacts with Protrudin and Rab7 at ER-late endosome membrane contact sites associated with mitochondria. | ||
Nat Commun Protrudin functions from the endoplasmic reticulum to support axon regeneration in the adult CNS. | ||
Nat Commun Selective EMC subunits act as molecular tethers of intracellular organelles exploited during viral entry. | ||
Proc Natl Acad Sci U S A Protrudin binds atlastins and endoplasmic reticulum-shaping proteins and regulates network formation.
| ||
Cell Mol Life Sci ESCRT-III-associated proteins and spastin inhibit protrudin-dependent polarised membrane traffic. | ||
Cell Mol Life Sci Protrudin regulates FAK activation, endothelial cell migration and angiogenesis.
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