验证数据展示
经过测试的应用
| Positive WB detected in | HUVEC cells, HSC-T6 cells, PC-12 cells, U2OS cells, A549 cells, HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
68038-1-Ig targets ZFP36L1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Rat samples.
| 经测试应用 | WB, IF/ICC, ELISA Application Description |
| 经测试反应性 | Human, Rat |
| 免疫原 |
CatNo: Ag30469 Product name: Recombinant human ZFP36L1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-338 aa of BC018340 Sequence: MTTTLVSATIFDLSEVLCKGNKMLNYSAPSAGGCLLDRKAVGTPAGGGFPRRHSVTLPSSKFHQNQLLSSLKGEPAPALSSRDSRFRDRSFSEGGERLLPTQKQPGGGQVNSSRYKTELCRPFEENGACKYGDKCQFAHGIHELRSLTRHPKYKTELCRTFHTIGFCPYGPRCHFIHNAEERRALAGARDLSADRPRLQHSFSFAGFPSAAATAAATGLLDSPTSITPPPILSADDLLGSPTLPDGTNNPFAFSSQELASLFAPSMGLPGGGSPTTFLFRPMSESPHMFDSPPSPQDSLSDQEGYLSSSSSSHSGSDSPTLDNSRRLPIFSRLSISDD 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG2b |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | zinc finger protein 36, C3H type-like 1 |
| 别名 | Berg36, BRF1, Butyrate response factor 1, cMG1, EGF response factor 1, ERF 1, ERF1, Protein TIS11B, RNF162B, TIS11B, ZFP36L1 |
| 计算分子量 | 338 aa, 36 kDa |
| 观测分子量 | 36 kDa |
| GenBank蛋白编号 | BC018340 |
| 基因名称 | ZFP36L1 |
| Gene ID (NCBI) | 677 |
| RRID | AB_2918780 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q07352 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
As a member of the Tristetraprolin (TTP) RNA binding protein family, ZFP36L1 regulate gene expression post-transcriptionally by promoting mRNA decay. In mammals, ZFP36L1 and its family members have been shown to regulate development, cell diferentiation, cell cycle, apoptosis and anti-cancer capability by targeting an extensive overlapping repertoire of mRNAs.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for ZFP36L1 antibody 68038-1-Ig | Download protocol |
| WB protocol for ZFP36L1 antibody 68038-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
