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验证数据展示

  • WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

    WB analysis using 66279-1-Ig (same clone as 66279-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66279-1-Ig (ZEB1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    IHC staining of human colon cancer using 66279-1-Ig (same clone as 66279-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66279-1-Ig (ZEB1 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66279-1-PBS in a different storage buffer formulation.

ZEB1 Monoclonal antibody, PBS Only

ZEB1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66279-1-PBS

产品说明书

CloneNo. 1H1F1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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产品信息

66279-1-PBS targets ZEB1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 ZEB1 fusion protein Ag17118 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger E-box binding homeobox 1
别名 1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8
计算分子量 1125 aa, 124 kDa
观测分子量 190-210 kDa
GenBank蛋白编号BC112392
基因名称 ZEB1
Gene ID (NCBI) 6935
RRIDAB_2881662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP37275
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.