ZEB1 Monoclonal antibody

ZEB1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 66279-1-Ig

产品说明书

CloneNo. 1H1F1

宿主/亚型

Mouse / IgG1

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA and More (1)

1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, HeLa cells, Jurkat cells, SKOV-3 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66279-1-Ig targets ZEB1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 human
文献引用反应性human, mouse
免疫原 ZEB1 fusion protein Ag17118 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 zinc finger E-box binding homeobox 1
别名 1H1F1, AREB6, NIL2A, NIL-2-A zinc finger protein, TCF8
计算分子量 1125 aa, 124 kDa
观测分子量 190-210 kDa
GenBank蛋白编号BC112392
基因名称 ZEB1
Gene ID (NCBI) 6935
RRIDAB_2881662
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP37275
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZEB1(zinc-finger-enhancer binding protein 1), which is a member of the Zinc finger-Homeobox (ZFH) family of repressors, acts as a transcriptional repressor that inhibits interleukin-2 (IL-2) gene expression. Depending on the quantity of cDNA and on the cell type, ZEB1 can enhance or repress the promoter activity of the ATP1A1 gene. Represses E-cadherin promoter and induces an epithelial-mesenchymal transition (EMT) by recruiting SMARCA4/BRG1. Represses BCL6 transcription in the presence of the corepressor CTBP1. Positively regulates neuronal differentiation. The calculated molecular weight of ZEB1 is 124 kDa, but the post-phosphorylation of protein is about 190-210 kDa.

实验方案

Product Specific Protocols
WB protocol for ZEB1 antibody 66279-1-IgDownload protocol
IHC protocol for ZEB1 antibody 66279-1-IgDownload protocol
IF protocol for ZEB1 antibody 66279-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Theranostics

FSH induces EMT in ovarian cancer via ALKBH5-regulated Snail m6A demethylation

Authors - Xingyan Xu
humanWB

Biochim Biophys Acta Mol Cell Res

The calcium pump PMCA4 prevents epithelial-mesenchymal transition by inhibiting NFATc1-ZEB1 pathway in gastric cancer.

Authors - Teng Wang
humanWB

Breast Cancer

Overexpression of SERPINA3 promotes tumor invasion and migration, epithelial-mesenchymal-transition in triple-negative breast cancer cells.

Authors - Yingzi Zhang
humanWB

J Clin Med

A Mutation in ZNF143 as a Novel Candidate Gene for Endothelial Corneal Dystrophy.

Authors - Yonggoo Kim
humanWB

Front Oncol

Identification of hub genes within the CCL18 signaling pathway in hepatocellular carcinoma through bioinformatics analysis

Authors - Jinlei Mao
mouseIF

Am J Physiol Lung Cell Mol Physiol

NFkB/RelA Signaling in Secretoglobin Progenitors Mediates Plasticity and MMP-Induced Barrier Disruption In House Dust Mite-Induced Allergic Asthma

Authors - Melissa E Skibba
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