ZC3H4 Polyclonal antibody
ZC3H4 Polyclonal Antibody for IF/ICC, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC, IHC, WB,ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, human brain tissue |
| Positive IHC detected in | human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | Hela cells |
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF) | IF : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 9 publications below |
| IF | See 3 publications below |
产品信息
20041-1-AP targets ZC3H4 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Applications | IF/ICC, IHC, WB,ELISA |
| Cited Applications | WB, IF |
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Immunogen | Peptide 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | zinc finger CCCH-type containing 4 |
| Synonyms | C19orf7, KIAA1064, ZC3H4 |
| Calculated Molecular Weight | 140 kDa |
| Observed Molecular Weight | 130-140 kDa |
| GenBank Accession Number | NM_015168 |
| Gene Symbol | ZC3H4 |
| Gene ID (NCBI) | 23211 |
| RRID | AB_10667456 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9UPT8 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ZC3H4, also named as C19orf7 and KIAA1064, is a 140kd protein. The antibody can recognizes near the C-term of ZC3H4.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IHC protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| IF protocol for ZC3H4 antibody 20041-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Macromol CircDYM attenuates microglial apoptosis via CEBPB/ZC3H4 axis in LPS-induced mouse model of depression | ||
FASEB J Silica-induced initiation of circular ZC3H4 RNA/ZC3H4 pathway promotes the pulmonary macrophage activation. | ||
Cell Biosci A missing piece of the puzzle in pulmonary fibrosis: anoikis resistance promotes fibroblast activation. | ||
Lab Invest Elk-1 transcriptionally regulates ZC3H4 expression to promote silica-induced epithelial-mesenchymal transition. | ||
Toxicol Appl Pharmacol ZC3H4 promotes pulmonary fibrosis via an ER stress-related positive feedback loop. | ||
Environ Toxicol Pharmacol ZC3H4 mediates silica-induced EndoMT via ER stress and autophagy. |
