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验证数据展示

  • WB analysis using 17611-1-AP (same clone as 17611-1-PBS)

    WB analysis using 17611-1-AP (same clone as 17611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17611-1-AP (ZBTB4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17611-1-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 17611-1-AP (same clone as 17611-1-PBS)

    WB analysis of PC-3 using 17611-1-AP (same clone as 17611-1-PBS)

    WB result of ZBTB4 antibody (17611-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-ZBTB4 transfected PC-3 cells. This data was developed using the same antibody clone with 17611-1-PBS in a different storage buffer formulation.

ZBTB4 Polyclonal antibody, PBS Only

ZBTB4 Polyclonal Antibody for WB, Indirect ELISA
Cat No. 17611-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, Indirect ELISA

别名

KAISO L1, KAISO-L1, KAISO-like zinc finger protein 1, ZBTB 4, Zinc finger and BTB domain-containing protein 4

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

17611-1-PBS targets ZBTB4 in WB, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag11774

Product name: Recombinant human ZBTB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-353 aa of BC043352

Sequence: MPPPAEVTDPSHAPAVLRQLNEQRLRGLFCDVTLIAGDTKFPAHRSVLAASSPFFREALLTSAPLPLPPATGGAAPNPATTTAASSSSSSSSSSSSSSSSASSSSSSSSSSPPPASPPASSPPRVLELPGVPAAAFSDVLNFIYSARLALPGGGGDGAAVAEIGALGRRLGISRLQGLGEGGDAWVPSTPAPMATSQPEEDSFGPGPRPAGEWEGDRAEAQAPDLQCSLPRRPLPCPQCGKSFIHPKRLQTHEAQCRRGASTRGSTGLGAGGAGPGGPAGVDASALPPPVGFRGGPEHVVKVVGGHVLYVCAACERSYVTLSSLKRHSNVHSWRRKYPCRYCEKVFALAEYRT

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 4
别名 KAISO L1, KAISO-L1, KAISO-like zinc finger protein 1, ZBTB 4, Zinc finger and BTB domain-containing protein 4
计算分子量 1013 aa, 105 kDa
观测分子量105-140 kDa
GenBank蛋白编号BC043352
基因名称 ZBTB4
Gene ID (NCBI) 57659
RRIDAB_3741925
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9P1Z0
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. The majority of zinc-finger proteins contain a Krüppel-type DNA binding domain and a KRAB domain, which is thought to interact with KAP1, thereby recruiting histone modifying proteins. ZBTB4 (zinc finger and BTB domain containing 4), also known as KAISO-L1 (KAISO-like zinc finger protein 1), is a 1,013 amino acid nuclear protein that is involved in transcriptional regulation. ZBTB4 contains one BTB (POZ) domain, six C2H2-type zinc fingers and is phosphorylated and downregulated by HIPK2. The gene encoding ZBTB4 maps to human chromosome 17, which comprises over 2.5% of the human genome and encodes over 1,200 genes.

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