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验证数据展示

  • WB analysis of A431 using 55270-1-AP (same clone as 55270-1-PBS)

    WB analysis of A431 using 55270-1-AP (same clone as 55270-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 55270-1-AP (ZBTB33 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

  • IP experiment of A431 using 55270-1-AP (same clone as 55270-1-PBS)

    IP experiment of A431 using 55270-1-AP (same clone as 55270-1-PBS)

    IP result of anti-ZBTB33 (IP:55270-1-AP, 4ug; Detection:55270-1-AP 1:300) with A431 cells lysate 1200ug. This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary cancer using 55270-1-AP (same clone as 55270-1-PBS)

    IHC staining of human ovary cancer using 55270-1-AP (same clone as 55270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZBTB33 antibody included
  • IHC staining of human intrahepatic cholangiocarcinoma using 55270-1-AP (same clone as 55270-1-PBS)

    IHC staining of human intrahepatic cholangiocarcinoma using 55270-1-AP (same clone as 55270-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 55270-1-AP (ZBTB33 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 55270-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ZBTB33 antibody included

ZBTB33 Polyclonal antibody, PBS Only

ZBTB33 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 55270-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IP, Indirect ELISA

别名

ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy ZBTB33 Ready-To-Use IHC Kit. ZBTB33 primary antibody included.

产品信息

55270-1-PBS targets ZBTB33 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human
免疫原

Peptide

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zinc finger and BTB domain containing 33
别名 ZNF348, ZNF kaiso, Zinc finger and BTB domain-containing protein 33, Transcriptional regulator Kaiso, KAISO
计算分子量 74 kDa
观测分子量 68-74 kDa
GenBank蛋白编号NM_006777
基因名称 ZBTB33
Gene ID (NCBI) 10009
RRIDAB_2881295
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ86T24
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)

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