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ZBTB33 Polyclonal antibody

ZBTB33 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IP, WB,ELISA and More (4)

Conjugate

Unconjugated

Cat No : 55270-1-AP

Print datasheet

Synonyms

KAISO, ZBTB33, ZNF kaiso, ZNF348

验证数据展示

  • WB analysis of A431 using 55270-1-AP

    WB analysis of A431 using 55270-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 55270-1-AP (ZBTB33 Antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • IP experiment of A431 using 55270-1-AP

    IP experiment of A431 using 55270-1-AP

    IP result of anti-ZBTB33 (IP:55270-1-AP, 4ug; Detection:55270-1-AP 1:300) with A431 cells lysate 1200ug.

经过测试的应用

Positive WB detected inA431 cells
Positive IP detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy ZBTB33 Ready-To-Use IHC Kit. ZBTB33 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

55270-1-AP targets ZBTB33 in WB, IP, IF, IHC, CoIP, ChIP, ELISA applications and shows reactivity with human samples.

Tested Applications IP, WB,ELISA
Cited ApplicationsIF, IHC, CoIP, ChIP
Tested Reactivity human
Cited Reactivityhuman
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name zinc finger and BTB domain containing 33
Synonyms KAISO, ZBTB33, ZNF kaiso, ZNF348
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 68-74 kDa
GenBank Accession NumberNM_006777
Gene Symbol ZBTB33
Gene ID (NCBI) 10009
RRIDAB_2881295
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ86T24
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZBTB33, also named as Transcriptional regulator Kaiso, is a 672 amino acid protein, which localizes in nucleus and cytoplasm. ZBTB33 as a transcriptional regulator with bimodal DNA-binding specificity binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). ZBTB33 recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters and may contribute to the repression of target genes of the Wnt signaling pathway. The calculated molecular weight of ZBTB33 is 75 kDa, but modified protein is about 100 kDa (PMID: 10207085 and PMID: 16354691)

实验方案

Product Specific Protocols
WB protocol for ZBTB33 antibody 55270-1-APDownload protocol
IP protocol for ZBTB33 antibody 55270-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Front Immunol

Diagnostic value of anti-Kaiso autoantibody in axial spondyloarthritis

Authors - Xinzhe Feng
View Article
humanCoIP,ChIP

Biochem Biophys Res Commun

ZNF131 facilitates the growth of hepatocellular carcinoma by acting as a transcriptional activator of SMC4 expression

Authors - Meng Jin
View Article