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验证数据展示

  • IHC staining of human liver cancer using 13285-1-AP (same clone as 13285-1-PBS)

    IHC staining of human liver cancer using 13285-1-AP (same clone as 13285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13285-1-AP (same clone as 13285-1-PBS)

    IF Staining of HeLa using 13285-1-AP (same clone as 13285-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ZBP1 antibody (13285-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

    IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

    IHC staining of human colon cancer using 13285-1-AP (same clone as 13285-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 13285-1-AP (ZBP1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

  • WB analysis using 13285-1-AP (same clone as 13285-1-PBS)

    WB analysis using 13285-1-AP (same clone as 13285-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 13285-1-AP (ZBP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13285-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

ZBP1 Polyclonal antibody, PBS Only

ZBP1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 13285-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

C20orf183, DAI, DLM 1, DLM1, Tumor stroma and activated macrophage protein DLM-1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13285-1-PBS targets ZBP1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag4119

Product name: Recombinant human ZBP1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-149 aa of BC028218

Sequence: MAQAPADPGREGHLEQRILQVLTEAGSPVKLAQLVKECQAPKRELNQVLYRMKKELKVSLTSPATWCLGGTDPEGEGPAELALSSPGNCHPGEAGLTLQGASWQWTSTDLSLGSNLNSATWELTGFLSLCLGFFFWLMELTAGLLGRGC

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 Z-DNA binding protein 1
别名 C20orf183, DAI, DLM 1, DLM1, Tumor stroma and activated macrophage protein DLM-1
计算分子量 46 kDa
观测分子量 42-68 kDa
GenBank蛋白编号BC028218
基因名称 ZBP1
Gene ID (NCBI) 81030
RRIDAB_2122651
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H171
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZBP1(Z-DNA-binding protein 1), also named as DAI or DLM-1, is a nucleic acid sensor with some isoforms. It has been reported that ZBP1 can sense the viral nucleic acids of viruses and induce the death of infected cells to prevent virus transmission. ZBP1 can be activated and causes necrocytosis without viral infection, which is associated with nuclear Z-form nucleic acids. ZBP1 can be detected 42 kDa, 55 kDa, 68 kDa isoforms (PMID: 37012234, PMID: 9121465).

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