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验证数据展示

  • WB analysis using 33956-1-AP (same clone as 33956-1-PBS)

    WB analysis using 33956-1-AP (same clone as 33956-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33956-1-AP (ZAN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33956-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse testis using 33956-1-AP (same clone as 33956-1-PBS)

    IP experiment of mouse testis using 33956-1-AP (same clone as 33956-1-PBS)

    IP result of anti-ZAN (IP:33956-1-AP, 4ug; Detection:33956-1-AP 1:800) with mouse testis tissue lysate 1520 ug. This data was developed using the same antibody clone with 33956-1-PBS in a different storage buffer formulation.

ZAN Polyclonal antibody, PBS Only

ZAN Polyclonal Antibody for WB, IP, Indirect ELISA
Cat No. 33956-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, Indirect ELISA

别名

Zonadhesin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33956-1-PBS targets ZAN in WB, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35843

Product name: Recombinant human ZAN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 18-200 aa of NM_003386

Sequence: RKEKPPDQKLVVRSSRDNYVLTQCDFEDDAKPLCDWSQVSADDEDWVRASGPSPTGSTGAPGGYPNGEGSYLHMESNSFHRGGVARLLSPDLWEQGPLCVHFAHHMFGLSWGAQLRLLLLSGEEGRRPDVLWKHWNTQRPSWMLTTVTVPAGFTLPTRLMFEGTRGSTAYLDIALDALSIRRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zonadhesin
别名 Zonadhesin
计算分子量305kDa
观测分子量230 kDa
GenBank蛋白编号NM_003386
基因名称 ZAN
Gene ID (NCBI) 7455
RRIDAB_3743123
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y493
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ZAN (Zonadhesin) is a sperm-specific type-I transmembrane glycoprotein essential for species-restricted binding between spermatozoa and the egg's zona pellucida. First isolated from boar sperm, it is expressed solely in post-meiotic male germ cells (early spermatids) and localises to the apical segment of the sperm head.

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