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验证数据展示

  • WB analysis using 33956-1-AP

    WB analysis using 33956-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 33956-1-AP (ZAN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • IP experiment of mouse testis using 33956-1-AP

    IP experiment of mouse testis using 33956-1-AP

    IP result of anti-ZAN (IP:33956-1-AP, 4ug; Detection:33956-1-AP 1:800) with mouse testis tissue lysate 1520 ug.

ZAN Polyclonal antibody

ZAN Polyclonal Antibody for WB, IP, ELISA
Cat No. 33956-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IP, ELISA

别名

Zonadhesin

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, mouse testis tissue
Positive IP detected inmouse testis tissue

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

33956-1-AP targets ZAN in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IP, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag35843

Product name: Recombinant human ZAN protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 18-200 aa of NM_003386

Sequence: RKEKPPDQKLVVRSSRDNYVLTQCDFEDDAKPLCDWSQVSADDEDWVRASGPSPTGSTGAPGGYPNGEGSYLHMESNSFHRGGVARLLSPDLWEQGPLCVHFAHHMFGLSWGAQLRLLLLSGEEGRRPDVLWKHWNTQRPSWMLTTVTVPAGFTLPTRLMFEGTRGSTAYLDIALDALSIRRG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 zonadhesin
别名 Zonadhesin
计算分子量305kDa
观测分子量230 kDa
GenBank蛋白编号NM_003386
基因名称 ZAN
Gene ID (NCBI) 7455
RRIDAB_3743123
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9Y493
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ZAN (Zonadhesin) is a sperm-specific type-I transmembrane glycoprotein essential for species-restricted binding between spermatozoa and the egg's zona pellucida. First isolated from boar sperm, it is expressed solely in post-meiotic male germ cells (early spermatids) and localises to the apical segment of the sperm head.

实验方案

Product Specific Protocols
IP protocol for ZAN antibody 33956-1-APDownload protocol
WB protocol for ZAN antibody 33956-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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