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验证数据展示

  • ChIP experiment of K-562 using 82712-3-RR (same clone as 82712-3-PBS)

    ChIP experiment of K-562 using 82712-3-RR (same clone as 82712-3-PBS)

    Chromatin was prepared from K-562 cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of YY1 (82712-3-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • WB analysis using 82712-3-RR (same clone as 82712-3-PBS)

    WB analysis using 82712-3-RR (same clone as 82712-3-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82712-3-RR (YY1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82712-3-RR (same clone as 82712-3-PBS)

    IF Staining of HepG2 using 82712-3-RR (same clone as 82712-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 82712-3-RR (same clone as 82712-3-PBS)

    IF Staining of HepG2 using 82712-3-RR (same clone as 82712-3-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YY1 antibody (82712-3-RR, Clone: 1B5 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), Alpha Tubulin antibody (66031-1-Ig, Clone: 1E4C11, red). This data was developed using the same antibody clone with 82712-3-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 82712-3-RR

    Affinity and Kinetic Characterization of 82712-3-RR

    Biolayer interferometry (BLl) kinetic assays of 82712-3-RR against Human YY1 were performed. The affinity constant is 0.835 nM.

YY1 Recombinant monoclonal antibody, PBS Only

YY1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA, ChIP-qPCR
Cat No. 82712-3-PBS

产品说明书

CloneNo. 1B5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, Indirect ELISA, ChIP-qPCR

别名

1B5, DELTA, Delta transcription factor, INO 80S, INO80S

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy YY1 Ready-To-Use IHC Kit. YY1 primary antibody included.

产品信息

82712-3-PBS targets YY1 in WB, IF/ICC, Indirect ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, Indirect ELISA, ChIP-qPCR Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag17792

Product name: Recombinant human YY1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-414 aa of BC037308

Sequence: MASGDTLYIATDGSEMPAEIVELHEIEVETIPVETIETTVVGEEEEEDDDDEDGGGGDHGGGGGHGHAGHHHHHHHHHHHPPMIALQPLVTDDPTQVHHHQEVILVQTREEVVGGDDSDGLRAEDGFEDQILIPVPAPAGGDDDYIEQTLVTVAAAGKSGGGGSSSSGGGRVKKGGGKKSGKKSYLSGGAGAAGGGGADPGNKKWEQKQVQIKTLEGEFSVTMWSSDEKKDIDHETVVEEQIIGENSPPDYSEYMTGKKLPPGGIPGIDLSDPKQLAEFARMKPRKIKEDDAPRTIACPHKGCTKMFRDNSAMRKHLHTHGPRVHVCAECGKAFVESSKLKRHQLVHTGEKPFQCTFEGCGKRFSLDFNLRTHVRIHTGDRPYVCPFDGCNKKFAQSTNLKSHILTHAKAKNNQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 YY1 transcription factor
别名 1B5, DELTA, Delta transcription factor, INO 80S, INO80S
计算分子量 414 aa, 45 kDa
观测分子量65-70 kDa
GenBank蛋白编号BC037308
基因名称 YY1
Gene ID (NCBI) 7528
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP25490
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YY1, also named as DELTA, INO80S and NF-E1, contains four C2H2-type zinc fingers and belongs to the YY transcription factor family. YY1 is a multifunctional transcription factor that exhibits positive and negative control on a large number of cellular and viral genes by binding to sites overlapping the transcription start site. YY1 may direct histone deacetylases and histone acetyltransferases to a promoter in order to activate or repress the promoter, thus implicating histone modification in the YY1. The open reading frame of the human YY1 cDNA encodes a protein of 414 amino acids with a predicted molecular weight of 44 kDa. However, YY1 migrates on SDS gels as a 65-68 kDa protein, probably due to the structure of the protein. It is a ubiquitously expressed transcription factor with fundamental roles in embryogenesis, differentiation, replication and proliferation.

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