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验证数据展示

  • IF Staining of MCF-7 using CL488-67698

    IF Staining of MCF-7 using CL488-67698

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 YWHAZ antibody (CL488-67698, Clone: 3G12B10 ) at dilution of 1:200.

  • FC experiment of MCF-7 using CL488-67698

    FC experiment of MCF-7 using CL488-67698

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated YWHAZ Monoclonal antibody (CL488-67698, Clone:3G12B10)(red), or 0.4 ug Isotype Control (CL488-66360-1) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated YWHAZ Monoclonal antibody

YWHAZ Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-67698

产品说明书

CloneNo. 3G12B10

宿主/亚型

Mouse / IgM

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

KCIP-1, KCIP 1, 3G12B10, 14-3-3 protein zeta/delta, 14-3-3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Mouse monoclonal antibodies of IgM isotype can be detected with "anti-mouse IgG (H+L)" secondary antibodies.

Planning an IHC experiment? We recommend our IHCeasy YWHAZ Ready-To-Use IHC Kit. YWHAZ primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67698-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67698 targets YWHAZ in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 YWHAZ fusion protein Ag6667 种属同源性预测
宿主/亚型 Mouse / IgM
抗体类别 Monoclonal
产品类型 Antibody
全称 tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide
别名 KCIP-1, KCIP 1, 3G12B10, 14-3-3 protein zeta/delta, 14-3-3
计算分子量 28 kDa
观测分子量28-30 kDa
GenBank蛋白编号BC003623
基因名称 YWHAZ
Gene ID (NCBI) 7534
RRIDAB_3672959
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Caprylic acid/ammonium sulfate precipitation
UNIPROT IDP63104
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YWHAZ (also known as 14-3-3 zeta) is a member of 14-3-3 proteins which were the first phosphoserine/phosphothreonine-binding proteins to be discovered. 14-3-3 family members interact with a wide spectrum of proteins and possess diverse functions. Mammals express seven distinct 14-3-3 isoforms (gamma, epsilon, beta, zeta, sigma, theta, tau) that form multiple homo- and hetero- dimmers. 14-3-3 proteins display the highest expression levels in the brain, and have been implicated in several neurodegenerative diseases, including Alzheimer's disease and amyotrophic lateral sclerosis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 YWHAZ antibody CL488-67698Download protocol
FC protocol for CL Plus 488 YWHAZ antibody CL488-67698Download protocol
Standard Protocols
Click here to view our Standard Protocols