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Conjugate
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验证数据展示

  • WB analysis using 68416-1-Ig

    WB analysis using 68416-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68416-1-Ig (YTHDF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis using 68416-1-Ig

    WB analysis using 68416-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68416-1-Ig (YTHDF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

YTHDF1 / YTHDF2 Monoclonal antibody

YTHDF1 / YTHDF2 Monoclonal Antibody for WB, ELISA
Cat No. 68416-1-Ig

产品说明书

CloneNo. 2F5H2

宿主/亚型

Mouse / IgG2a

种属反应性

Human, mouse , rat

应用

WB, ELISA

别名

CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, 4T1 cells, HSC-T6 cells
Planning an IHC experiment? We recommend our IHCeasy YTHDF2 Ready-To-Use IHC Kit. YTHDF2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68416-1-Ig targets YTHDF1 / YTHDF2 in WB, ELISA applications and shows reactivity with Human, mouse , rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 Human, mouse , rat
免疫原 YTHDF1 / YTHDF2 fusion protein Ag5922 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 YTH domain family, member 2
别名 CLL associated antigen KW 14, HGRG8, NY REN 2, YTH domain family protein 2, YTH domain family, member 2, YTHDF2
计算分子量 62 kDa
观测分子量63 kDa, 70 kDa
GenBank蛋白编号BC002559
基因名称 YTHDF2
Gene ID (NCBI) 51441
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9Y5A9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

YTHDF2, also named as YTH domain-containing family protein 2, is a 579 amino acid protein, which contains 1 YTH domain. YTHDF2 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs, and regulates mRNA stability. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF2 acts as a regulator of mRNA stability: binding to m6A-containing mRNAs results in the localization to mRNA decay sites, such as processing bodies (P-bodies), leading to mRNA degradation. YTHDF2 also acts as a promoter of cap-independent mRNA translation following heat shock stress: upon stress, relocalizes to the nucleus and specifically binds mRNAs with some m6A methylation mark at their 5'-UTR, protecting demethylation of mRNAs by FTO, thereby promoting cap-independent mRNA translation. The calculated molecular weight of YTHDF2 is 62 kDa, but the phosphorylated YTHDF2 is about 65-70 kDa.

实验方案

Product Specific Protocols
WB protocol for YTHDF1 / YTHDF2 antibody 68416-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols