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验证数据展示

  • WB analysis of HepG2 using 66745-1-Ig (same clone as 66745-1-PBS)

    WB analysis of HepG2 using 66745-1-Ig (same clone as 66745-1-PBS)

    WB result of YTHDF1 antibody (66745-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YTHDF1 transfected HepG2 cells. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • WB analysis using 66745-1-Ig (same clone as 66745-1-PBS)

    WB analysis using 66745-1-Ig (same clone as 66745-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of human breast cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of mouse testis using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of human lung cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of rat testis using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • IP experiment of Jurkat using 66745-1-Ig (same clone as 66745-1-PBS)

    IP experiment of Jurkat using 66745-1-Ig (same clone as 66745-1-PBS)

    IP result of anti-YTHDF1 (IP:66745-1-Ig, 4ug; Detection:66745-1-Ig 1:2000) with Jurkat cells lysate 2000 ug. This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    IHC staining of human colon cancer using 66745-1-Ig (same clone as 66745-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66745-1-Ig (YTHDF1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66745-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with YTHDF1 antibody included
  • Featured Product
  • KD/KO Validated

YTHDF1 Monoclonal antibody, PBS Only

YTHDF1 Monoclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 66745-1-PBS

产品说明书

CloneNo. 3A2H12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

3A2H12, C20orf21, DACA-1, DF1, YTH domain-containing family protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy YTHDF1 Ready-To-Use IHC Kit. YTHDF1 primary antibody included.

产品信息

66745-1-PBS targets YTHDF1 in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag11633

Product name: Recombinant human YTHDF1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 210-559 aa of BC050284

Sequence: VALTGVLSGNGGTNVNMPVSKPTSWAAIASKPAKPQPKMKTKSGPVMGGGLPPPPIKHNMDIGTWDNKGPVPKAPVPQQAPSPQAAPQPQQVAQPLPAQPPALAQPQYQSPQQPPQTRWVAPRNRNAAFGQSGGAGSDSNSPGNVQPNSAPSVESHPVLEKLKAAHSYNPKEFEWNLKSGRVFIIKSYSEDDIHRSIKYSIWCSTEHGNKRLDSAFRCMSSKGPVYLLFSVNGSGHFCGVAEMKSPVDYGTSAGVWSQDKWKGKFDVQWIFVKDVPNNQLRHIRLENNDNKPVTNSRDTQEVPLEKAKQVLKIISSYKHTTSIFDDFAHYEKRQEEEEVVRKERQSRNKQ

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 YTH domain family, member 1
别名 3A2H12, C20orf21, DACA-1, DF1, YTH domain-containing family protein 1
计算分子量 559 aa, 61 kDa
观测分子量 60 kDa
GenBank蛋白编号BC050284
基因名称 YTHDF1
Gene ID (NCBI) 54915
RRIDAB_2882093
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ9BYJ9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.

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