验证数据展示
经过测试的应用
| Positive WB detected in | HEK-293 cells, HepG2 cells, HeLa cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cellls |
| Positive IP detected in | Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human breast cancer tissue, mouse testis tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66745-1-Ig targets YTHDF1 in WB, IHC, IF, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF, CoIP, RIP |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, pig |
| 免疫原 | YTHDF1 fusion protein Ag11633 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | YTH domain family, member 1 |
| 别名 | YTH domain-containing family protein 1, DF1, DACA-1, C20orf21, 3A2H12 |
| 计算分子量 | 559 aa, 61 kDa |
| 观测分子量 | 60 kDa |
| GenBank蛋白编号 | BC050284 |
| 基因名称 | YTHDF1 |
| Gene ID (NCBI) | 54915 |
| RRID | AB_2882093 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | Q9BYJ9 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YTHDF1, also named as YTH domain-containing family protein 1 or C20orf21, is a 559 amino acid protein, which localizes in the cytoplasm. YTHDF1 specifically recognizes and binds N6-methyladenosine (m6A)-containing mRNAs, and promotes mRNA translation efficiency. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. YTHDF1 acts as a regulator of mRNA translation efficiency: promotes ribosome loading to m6A-containing mRNAs and interacts with translation initiation factors eIF3 (EIF3A or EIF3B) to facilitate translation initiation. YTHDF1 exists two isoform and calculated molecular weight of isoforms are 61 kDa and 21 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YTHDF1 antibody 66745-1-Ig | Download protocol |
| IHC protocol for YTHDF1 antibody 66745-1-Ig | Download protocol |
| IP protocol for YTHDF1 antibody 66745-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Angew Chem Int Ed Engl Small molecule-inducible and photoactivatable cellular RNA N1-methyladenosine editing | ||
Nucleic Acids Res The N6-methyladenosine RNA-binding protein YTHDF1 modulates the translation of TRAF6 to mediate the intestinal immune response.
| ||
Cancer Res RUVBL1/2 Blockade Targets YTHDF1 Activity to Suppress m6A-Dependent Oncogenic Translation and Colorectal Tumorigenesis
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EMBO Rep Severe fever with thrombocytopenia syndrome virus induces lactylation of m6A reader protein YTHDF1 to facilitate viral replication | ||
EMBO Rep N(6)-methyladenosine-binding protein YTHDF1 suppresses EBV replication and promotes EBV RNA decay.
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Int J Mol Sci Yth m6A RNA-Binding Protein 1 Regulates Osteogenesis of MC3T3-E1 Cells under Hypoxia via Translational Control of Thrombospondin-1
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