验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, mouse testis tissue, rat brain tissue |
| Positive IHC detected in | mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 5 publications below |
| IF | See 1 publications below |
产品信息
11730-1-AP targets YPEL5 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, zebrafish |
| 免疫原 |
CatNo: Ag2328 Product name: Recombinant human YPEL5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-121 aa of BC000836 Sequence: MGRIFLDHIGGTRLFSCANCDTILTNRSELISTRFTGATGRAFLFNKVVNLQYSEVQDRVMLTGRHMVRDVSCKNCNSKLGWIYEFATEDSQRYKEGRVILERALVRESEGFEEHVPSDNS 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | yippee-like 5 (Drosophila) |
| 别名 | CGI 127, Protein yippee like 5, yippee like 5 (Drosophila), YPEL5 |
| 计算分子量 | 121 aa, 14 kDa |
| 观测分子量 | 14 kDa |
| GenBank蛋白编号 | BC000836 |
| 基因名称 | YPEL5 |
| Gene ID (NCBI) | 51646 |
| RRID | AB_2217465 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P62699 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YPEL5(Protein yippee-like 5) belongs to the yippee family and is involved in a certain cell division-related function.During cell cycle, YPEL5 protein is detected at different subcellular localizations; at interphase, it is located in the nucleus and centrosome, then it changes location sequentially to spindle poles, mitotic spindle, and spindle midzone during mitosis, and finally transferrs to midbody at cytokinesis(PMID:20580816).
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YPEL5 antibody 11730-1-AP | Download protocol |
| IHC protocol for YPEL5 antibody 11730-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Neuron Molecular microcircuitry underlies functional specification in a basal ganglia circuit dedicated to vocal learning. | ||
Mol Oncol METTL3/YTHDF2 m6A axis accelerates colorectal carcinogenesis through epigenetically suppressing YPEL5. | ||
iScience Genome-wide CRISPR-Cas9 screen analyzed by SLIDER identifies network of repressor complexes that regulate TRIM24 | ||
bioRxiv Genome-wide screen of Mycobacterium tuberculosis- infected macrophages identified the GID/CTLH complex as a determinant of intracellular bacterial growth | ||
Nat Commun Genome-wide screen of Mycobacterium tuberculosis-infected macrophages revealed GID/CTLH complex-mediated modulation of bacterial growth |
