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验证数据展示

  • WB analysis of HeLa using 66551-1-Ig (same clone as 66551-1-PBS)

    WB analysis of HeLa using 66551-1-Ig (same clone as 66551-1-PBS)

    WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • WB analysis using 66551-1-Ig (same clone as 66551-1-PBS)

    WB analysis using 66551-1-Ig (same clone as 66551-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

YME1L1 Monoclonal antibody, PBS Only

YME1L1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66551-1-PBS

产品说明书

CloneNo. 2B7E4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2B7E4, ATP-dependent metalloprotease FtsH1, ATP-dependent zinc metalloprotease YME1L1, EC:3.4.24.-, FTSH

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66551-1-PBS targets YME1L1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2069

Product name: Recombinant human YME1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 348-716 aa of BC023507

Sequence: GSEFDEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKLEVR

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 YME1-like 1 (S. cerevisiae)
别名 2B7E4, ATP-dependent metalloprotease FtsH1, ATP-dependent zinc metalloprotease YME1L1, EC:3.4.24.-, FTSH
计算分子量 716 aa, 80 kDa
观测分子量 63 kDa
GenBank蛋白编号BC023507
基因名称 YME1L1
Gene ID (NCBI) 10730
RRIDAB_2881913
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ96TA2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

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