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验证数据展示

  • WB analysis of HeLa using 66551-1-Ig (same clone as 66551-1-PBS)

    WB analysis of HeLa using 66551-1-Ig (same clone as 66551-1-PBS)

    WB result of YME1L1 antibody (66551-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • WB analysis using 66551-1-Ig (same clone as 66551-1-PBS)

    WB analysis using 66551-1-Ig (same clone as 66551-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66551-1-Ig (YME1L1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    IHC staining of human kidney using 66551-1-Ig (same clone as 66551-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66551-1-Ig (YME1L1 antibody) at dilution of 1:300 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66551-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

YME1L1 Monoclonal antibody, PBS Only

YME1L1 Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 66551-1-PBS

产品说明书

CloneNo. 2B7E4

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, Indirect ELISA

别名

2B7E4, ATP-dependent metalloprotease FtsH1, ATP-dependent zinc metalloprotease YME1L1, EC:3.4.24.-, FTSH

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

66551-1-PBS targets YME1L1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 YME1L1 fusion protein Ag2069 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 YME1-like 1 (S. cerevisiae)
别名 2B7E4, ATP-dependent metalloprotease FtsH1, ATP-dependent zinc metalloprotease YME1L1, EC:3.4.24.-, FTSH
计算分子量 716 aa, 80 kDa
观测分子量 63 kDa
GenBank蛋白编号BC023507
基因名称 YME1L1
Gene ID (NCBI) 10730
RRIDAB_2881913
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ96TA2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).