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验证数据展示

  • WB analysis using 11510-1-AP (same clone as 11510-1-PBS)

    WB analysis using 11510-1-AP (same clone as 11510-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 11510-1-AP (YME1L1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 11510-1-AP (same clone as 11510-1-PBS)

    IP experiment of HeLa using 11510-1-AP (same clone as 11510-1-PBS)

    IP result of anti-YME1L1 (IP:11510-1-AP, 4ug; Detection:11510-1-AP 1:500) with HeLa cells lysate 1280 ug. This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 11510-1-AP (same clone as 11510-1-PBS)

    WB analysis of HeLa using 11510-1-AP (same clone as 11510-1-PBS)

    WB result of YME1L1 antibody (11510-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-YME1L1 transfected HeLa cells. This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 11510-1-AP (same clone as 11510-1-PBS)

    IF Staining of HepG2 using 11510-1-AP (same clone as 11510-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using YME1L1 antibody (11510-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 11510-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YME1L1 Polyclonal antibody, PBS Only

YME1L1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 11510-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

11510-1-PBS targets YME1L1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag2069

Product name: Recombinant human YME1L1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 348-716 aa of BC023507

Sequence: GSEFDEMFVGVGASRIRNLFREAKANAPCVIFIDELDSVGGKRIESPMHPYSRQTINQLLAEMDGFKPNEGVIIIGATNFPEALDNALIRPGRFDMQVTVPRPDVKGRTEILKWYLNKIKFDQSVDPEIIARGTVGFSGAELENLVNQAALKAAVDGKEMVTMKELEFSKDKILMGPERRSVEIDNKNKTITAYHESGHAIIAYYTKDAMPINKATIMPRGPTLGHVSLLPENDRWNETRAQLLAQMDVSMGGRVAEELIFGTDHITTGASSDFDNATKIAKRMVTKFGMSEKLGVMTYSDTGKLSPETQSAIEQEIRILLRDSYERAKHILKTHAKEHKNLAEALLTYETLDAKEIQIVLEGKKLEVR

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YME1-like 1 (S. cerevisiae)
别名
计算分子量 716 aa, 80 kDa
观测分子量63 kDa
GenBank蛋白编号BC023507
基因名称 YME1L1
Gene ID (NCBI) 10730
RRIDAB_2217459
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ96TA2
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YME1L1(ATP-dependent zinc metalloprotease) is also named as FTSH1, YME1L,Meg-4,PAMP. YME1L1 plays a phylogenetically conserved role in mitochondrial protein metabolism.It also ensures cell proliferation, maintains normal cristae morphology and complex I respiration activity, promotes antiapoptotic activity and protects mitochondria from the accumulation of oxidatively damaged membrane proteins. YME1L1 can prevent mitochondrial DNA inserting into nuclear. It has 3 isoforms produced by alternative splicing with the molecular weight of 86 kDa, 80 kDa and 76 kDa. This protein can migrate with a molecular weight of about 55-63 kDa, which is the size of the mature YME1L1 protein(PMID:22252130; 22354088).

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