验证数据展示
经过测试的应用
| Positive WB detected in | mouse colon tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
30243-1-AP targets YIPF6 in WB, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 |
CatNo: Ag32477 Product name: Recombinant human YIPF6 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 2-84 aa of BC012469 Sequence: AEAEESPGDPGTASPRPLFAGLSDISISQDIPVEGEITIPMRSRIREFDSSTLNESVRNTIMRDLKAVGKKFMHVLYPRKSNT 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Yip1 domain family, member 6 |
| 别名 | FinGER6, Protein YIPF6, Yip1 domain family, member 6, YIP1 family member 6 |
| 计算分子量 | 236 aa, 26 kDa |
| 观测分子量 | 55 kDa |
| GenBank蛋白编号 | BC012469 |
| 基因名称 | YIPF6 |
| Gene ID (NCBI) | 286451 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q96EC8 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YIPF6 encodes a Golgi- and ER-localized membrane protein that regulates vesicle trafficking and secretory pathway integrity through interaction with Rab GTPases. Highly expressed in intestinal epithelium and immune tissues, YIPF6 is essential for Paneth and goblet cell granule maturation and mucosal immune homeostasis. Loss of YIPF6 disrupts epithelial secretion and predisposes to inflammatory bowel-like disease, establishing it as a critical regulator of Golgi function and gut barrier defense.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YIPF6 antibody 30243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
