验证数据展示
经过测试的应用
| Positive WB detected in | SK-N-SH cells, mouse brain tissue, rat brain tissue |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
16406-1-AP targets YIF1B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human, mouse, rat |
| 免疫原 | YIF1B fusion protein Ag9538 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | Yip1 interacting factor homolog B (S. cerevisiae) |
| 别名 | YIP1-interacting factor homolog B, UNQ3073/PRO9905, Protein YIF1B, PP4519, FinGER8 |
| 计算分子量 | 294 aa, 32 kDa |
| 观测分子量 | 31~34 kDa |
| GenBank蛋白编号 | BC014974 |
| 基因名称 | YIF1B |
| Gene ID (NCBI) | 90522 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q5BJH7 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YIF1B is an intracellular membrane-bound protein belonging to the Yip family, a trafficking protein orthologue of YIF1P that was initially identified in Saccharomyces cerevisiae as interacting with YIP1P and essential for secretion. YIF1B is expressed in various tissues, especially in brain neurons (PMID: 33103737). YIF1B is localized in the intermediate compartment between the endoplasmic reticulum (ER) and the Golgi apparatus (PMID: 26077767). Western blot analysis detected YIF1B at an apparent molecular mass of 31 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YIF1B antibody 16406-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |