验证数据展示
经过测试的应用
| Positive WB detected in | A431 cells, HepG2 cells, A549 cells, DU 145 cells, HEK-293 cells |
| Positive IP detected in | A549 cells |
| Positive IHC detected in | human lung cancer tissue, human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Planning an IHC experiment? We recommend our IHCeasy YES1 Ready-To-Use IHC Kit. YES1 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 2 publications below |
| WB | See 5 publications below |
| IHC | See 2 publications below |
产品信息
20243-1-AP targets YES1-Specific in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IP, ELISA Application Description |
| 文献引用应用 | WB, IHC |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1 |
| 别名 | YES1, c yes, EC:2.7.10.2, HsT441, P61 YES |
| 计算分子量 | 61 kDa |
| 观测分子量 | 61 kDa |
| GenBank蛋白编号 | NM_005433 |
| 基因名称 | YES1 |
| Gene ID (NCBI) | 7525 |
| RRID | AB_10697656 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P07947 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate. The antibody is specific to YES1.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for YES1-Specific antibody 20243-1-AP | Download protocol |
| IHC protocol for YES1-Specific antibody 20243-1-AP | Download protocol |
| IP protocol for YES1-Specific antibody 20243-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Crit Care Med YES1 Drives Lung Cancer Growth and Progression and Predicts Sensitivity to Dasatinib. | ||
Sci Adv A brain proteomic signature of incipient Alzheimer's disease in young APOE ε4 carriers identifies novel drug targets. | ||
J Immunother Cancer SRC family kinase (SFK) inhibitor dasatinib improves the antitumor activity of anti-PD-1 in NSCLC models by inhibiting Treg cell conversion and proliferation.
| ||
Cell Death Dis Linc01133 contributes to gastric cancer growth by enhancing YES1-dependent YAP1 nuclear translocation via sponging miR-145-5p. | ||
J Cell Biochem miR-17-5p up-regulates YES1 to modulate the cell cycle progression and apoptosis in ovarian cancer cell lines.
| ||
iScience DAB2IP suppresses invadopodia formation through destabilizing ALK by interacting with USP10 in breast cancer |
