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验证数据展示

  • WB analysis of HEK-293 using 13980-1-AP (same clone as 13980-1-PBS)

    WB analysis of HEK-293 using 13980-1-AP (same clone as 13980-1-PBS)

    WB result of YAF2 antibody (13980-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-YAF2 transfected HEK-293 cells. This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 13980-1-AP (same clone as 13980-1-PBS)

    IP experiment of Jurkat using 13980-1-AP (same clone as 13980-1-PBS)

    IP result of anti-YAF2 (IP:13980-1-AP, 4ug; Detection:13980-1-AP 1:800) with Jurkat cells lysate 1920 ug. This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • IP experiment of HeLa using 13980-1-AP (same clone as 13980-1-PBS)

    IP experiment of HeLa using 13980-1-AP (same clone as 13980-1-PBS)

    IP result of anti-YAF2 (IP:13980-1-AP, 4ug; Detection:13980-1-AP 1:600) with HeLa cells lysate 1760 ug. This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13980-1-AP (same clone as 13980-1-PBS)

    IHC staining of mouse brain using 13980-1-AP (same clone as 13980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse brain using 13980-1-AP (same clone as 13980-1-PBS)

    IHC staining of mouse brain using 13980-1-AP (same clone as 13980-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13980-1-AP (YAF2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • IF Staining of U2OS using 13980-1-AP (same clone as 13980-1-PBS)

    IF Staining of U2OS using 13980-1-AP (same clone as 13980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using YAF2 antibody (13980-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 13980-1-AP (same clone as 13980-1-PBS)

    IF Staining of HeLa using 13980-1-AP (same clone as 13980-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using YAF2 antibody (13980-1-AP) at dilution of 1:800 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 13980-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

YAF2 Polyclonal antibody, PBS Only

YAF2 Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 13980-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, IF/ICC, IP, Indirect ELISA

别名

YY1 associated factor 2, YY1-associated factor 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13980-1-PBS targets YAF2 in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag5023

Product name: Recombinant human YAF2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-204 aa of BC037777

Sequence: MGDKKSPTRPKRQPKPSSDEGYWDCSVCTFRNSAEAFKCMMCDVRKGTSTRSTLFEVIVSASRTKEPLKFPISGRKPRPVSQLVAQQVTQQFVPPTQSKKEKKDKVEKEKSEKETTSKKNSHKKTRPRLKNVDRSSAQHLEVTVGDLTVIITDFKEKTKSPPASSAASADQHSQSGSSSDNTERGMSRSSSPRGEASSLNGESH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 YY1 associated factor 2
别名 YY1 associated factor 2, YY1-associated factor 2
计算分子量 204 aa, 23 kDa
观测分子量20-30 kDa
GenBank蛋白编号BC037777
基因名称 YAF2
Gene ID (NCBI) 10138
RRIDAB_3669173
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IY57
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

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