验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | U2OS cells, HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:200-1:1000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13980-1-AP targets YAF2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, IP, ELISA Application Description |
经测试反应性 | human, mouse |
免疫原 | YAF2 fusion protein Ag5023 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | YY1 associated factor 2 |
别名 | YY1-associated factor 2, YY1 associated factor 2 |
计算分子量 | 204 aa, 23 kDa |
观测分子量 | 20-30 kDa |
GenBank蛋白编号 | BC037777 |
基因名称 | YAF2 |
Gene ID (NCBI) | 10138 |
RRID | AB_3669173 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q8IY57 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).
实验方案
Product Specific Protocols | |
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WB protocol for YAF2 antibody 13980-1-AP | Download protocol |
IHC protocol for YAF2 antibody 13980-1-AP | Download protocol |
IF protocol for YAF2 antibody 13980-1-AP | Download protocol |
IP protocol for YAF2 antibody 13980-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |