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验证数据展示

  • WB analysis using 15655-1-AP

    WB analysis using 15655-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 15655-1-AP (XPNPEP3 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IP experiment of PC-3 using 15655-1-AP

    IP experiment of PC-3 using 15655-1-AP

    IP result of anti-XPNPEP3 (IP:15655-1-AP, 4ug; Detection:15655-1-AP 1:400) with PC-3 cells lysate 2000ug.

  • IHC staining of mouse liver using 15655-1-AP

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse liver using 15655-1-AP

    IHC staining of mouse liver using 15655-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 15655-1-AP (XPNPEP3 antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

XPNPEP3 Polyclonal antibody

XPNPEP3 Polyclonal Antibody for WB, IHC, IP, ELISA
Cat No. 15655-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, ELISA

别名

X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inA431 cells, human testis tissue, human spleen tissue, PC-3 cells, 4T1 cells, mouse liver
Positive IP detected inPC-3 cells
Positive IHC detected inmouse liver tissue, human oesophagus tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

15655-1-AP targets XPNPEP3 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag8007

Product name: Recombinant human XPNPEP3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 158-507 aa of BC004989

Sequence: PSRELWDGPRSGTDGAIALTGVDEAYTLEEFQHLLPKMKAETNMVWYDWMRPSHAQLHSDYMQPLTEAKAKSKNKVRGVQQLIQRLRLIKSPAEIERMQIAGKLTSQAFIETMFTSKAPVEEAFLYAKFEFECRARGADILAYPPVVAGGNRSNTLHYVKNNQLIKDGEMVLLDGGCESSCYVSDITRTWPVNGRFTAPQAELYEAVLEIQRDCLALCFPGTSLENIYSMMLTLIGQKLKDLGIMKNIKENNAFKAARKYCPHHVGHYLGMDVHDTPDMPRSLPLQPGMVITIEPGIYIPEDDKDAPEKFRGLGVRIEDDVVVTQDSPLILSADCPKEMNDIEQICSQAS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 X-prolyl aminopeptidase (aminopeptidase P) 3, putative
别名 X Pro aminopeptidase 3, EC:3.4.11.9, APP3m, APP3c, APP3
计算分子量 57 kDa
观测分子量 57 kDa
GenBank蛋白编号BC004989
基因名称 XPNPEP3
Gene ID (NCBI) 63929
RRIDAB_2288500
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NQH7
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XPNPEP3 gene encodes X-prolyl aminopeptidase 3 which functions to remove the penultimate N-terminal Proline residue from nascent proteins and appears to play a role in ciliary function. CRC tissue microarray revealed increased XPNPEP3 expression in tumor compared to matched normal samples (PMID:29383790). Other study found that XPNPEP3 localizes to mitochondria in renal cells in vitro and to kidney tubules in a cell type-specific pattern, and mutations in the gene are associated with NPHP-like disorder (PMID:20179356).

实验方案

Product Specific Protocols
WB protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IHC protocol for XPNPEP3 antibody 15655-1-APDownload protocol
IP protocol for XPNPEP3 antibody 15655-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols