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验证数据展示

  • WB analysis of human placenta using 27049-1-AP

    WB analysis of human placenta using 27049-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27049-1-AP (XKRX Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IHC staining of human placenta using 27049-1-AP

    IHC staining of human placenta using 27049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27049-1-AP (XKRX Antibody) at dilution of 1:200 (under 10x lens).

  • IHC staining of human placenta using 27049-1-AP

    IHC staining of human placenta using 27049-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 27049-1-AP (XKRX Antibody) at dilution of 1:200 (under 40x lens).

XKRX Polyclonal antibody

XKRX Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27049-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, ELISA

别名

Membrane protein XPLAC, X Kell blood group-related, X-linked, XK related protein 2, XKR2, XK-related protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27049-1-AP targets XKRX in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag25266

Product name: Recombinant human XKRX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 101-210 aa of BC137010

Sequence: VHRDLAKDKPLSLFMHLILLGPVIRCLEAMIKYLTLWKKEEQEEPYVSLTRKKMLIDGEEVLIEWEVGHSIRTLAMHRNAYKRMSQIQAFLGSVPQLTYQLYVSLISAEV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 XK, Kell blood group complex subunit-related, X-linked
别名 Membrane protein XPLAC, X Kell blood group-related, X-linked, XK related protein 2, XKR2, XK-related protein 2
计算分子量 462 aa, 54 kDa
观测分子量 30 kDa
GenBank蛋白编号BC137010
基因名称 XKRX
Gene ID (NCBI) 402415
RRIDAB_2880732
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ6PP77
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

XKRX (XK-associated X-linked protein) is a component of the XK/Kell complex in the Kell blood group system, encoding a membrane protein with multiple transmembrane domains that is exposed on the cell surface and may function as a membrane transport protein. It is expressed predominantly in the placenta and syncytiotrophoblasts. It has 2 isoforms, with molecular weights of 52 kDa and 28 kDa respectively. Its function has not been fully clarified yet.

实验方案

Product Specific Protocols
WB protocol for XKRX antibody 27049-1-APDownload protocol
IHC protocol for XKRX antibody 27049-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols