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验证数据展示

  • WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    WB analysis using 66800-1-Ig (same clone as 66800-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66800-1-Ig (XIAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB analysis of HEK-293 using 66800-1-Ig (same clone as 66800-1-PBS)

    WB result of XIAP antibody (66800-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XIAP transfected HEK-293 cells. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XIAP antibody included
  • IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    IHC staining of human colon using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66800-1-Ig (XIAP antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with XIAP antibody included
  • IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    IF Staining of human colon cancer using 66800-1-Ig (same clone as 66800-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using XIAP antibody (66800-1-Ig, Clone: 2A1A12 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    FC experiment of HeLa using 66800-1-Ig (same clone as 66800-1-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human XIAP (66800-1-Ig, Clone:2A1A12) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66800-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

XIAP Monoclonal antibody, PBS Only

XIAP Monoclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 66800-1-PBS

产品说明书

CloneNo. 2A1A12

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

2A1A12, API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy XIAP Ready-To-Use IHC Kit. XIAP primary antibody included.

产品信息

66800-1-PBS targets XIAP in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag18088

Product name: Recombinant human XIAP protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-497 aa of BC032729

Sequence: MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFANFPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAVDRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQSTNSGIQNGQYKVENYLGSRDHFALDRPSETHADYLLRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPDYAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNWEPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVSSDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVNKEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSEDPWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEECLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFSFKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKDSMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMDRNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITFKQKIFMS

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 X-linked inhibitor of apoptosis
别名 2A1A12, API3, Baculoviral IAP repeat-containing protein 4, E3 ubiquitin-protein ligase XIAP, EC:2.3.2.27
计算分子量 60 kDa
观测分子量 57 kDa
GenBank蛋白编号BC032729
基因名称 XIAP
Gene ID (NCBI) 331
RRIDAB_2882143
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP98170
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.

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