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XIAP Monoclonal antibody
XIAP Monoclonal Antibody for FC, IF-P, IHC, WB, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat and More (1)
Applications
FC, IF-P, IHC, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2A1A12
验证数据展示
经过测试的应用
| Positive WB detected in | LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, HEK-293T cells, Jurkat cells |
| Positive IHC detected in | human colon tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF detected in | human colon cancer tissue, human colon tissue |
| Positive FC detected in | HeLa cells |
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| Flow Cytometry (FC) | FC : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
66800-1-Ig targets XIAP in WB, IF, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.
| Tested Applications | FC, IF-P, IHC, WB, ELISA |
| Cited Applications | WB, IF, IHC |
| Tested Reactivity | Human, mouse, rat |
| Cited Reactivity | human, mouse |
| Immunogen | XIAP fusion protein Ag18088 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | X-linked inhibitor of apoptosis |
| Synonyms | API3, BIRC4, hIAP 3, hIAP3, hILP, IAP 3, IAP like protein, IAP3, ILP, ILP1, MIHA, X linked IAP, XIAP, XLP2 |
| Calculated Molecular Weight | 60 kDa |
| Observed Molecular Weight | 57 kDa |
| GenBank Accession Number | BC032729 |
| Gene Symbol | XIAP |
| Gene ID (NCBI) | 331 |
| RRID | AB_2882143 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P98170 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XIAP, also named as API3, BIRC4 and IAP3, belongs to the IAP family. It has E3 ubiquitin-protein ligase activity. It mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. XIAP is an inhibitor of caspase-3, -7 and -9. It mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B. XIAP is an apoptotic suppressor. It is ubiquitinated and degraded by the proteasome in apoptotic cells.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XIAP antibody 66800-1-Ig | Download protocol |
| IHC protocol for XIAP antibody 66800-1-Ig | Download protocol |
| IF protocol for XIAP antibody 66800-1-Ig | Download protocol |
| FC protocol for XIAP antibody 66800-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Inhibition of PI3K/AKT signaling via ROS regulation is involved in Rhein-induced apoptosis and enhancement of oxaliplatin sensitivity in pancreatic cancer cells. | ||
Oncol Lett PP2A promotes apoptosis and facilitates docetaxel sensitivity via the PP2A/p-eIF4B/XIAP signaling pathway in prostate cancer. | ||
Gene Study on the mechanism of S100A4-mediated cancer oncogenesis in uveal melanoma cells through the integration of bioinformatics and in vitro experiments | ||
Chem Biol Interact Hsa_circ_0005050 interacts with ILF3 and affects cell apoptosis and proliferation by disrupting the balance between p53 and p65 | ||
