验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| KD/KO | See 1 publications below |
| WB | See 21 publications below |
产品信息
55156-1-AP targets XDH in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, bovine |
| 免疫原 | Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | xanthine dehydrogenase |
| 别名 | XO, XDHA, XD, Xanthine oxidoreductase, Xanthine Oxidase |
| 计算分子量 | 146 kDa |
| 观测分子量 | 147-150 kDa |
| GenBank蛋白编号 | NM_000379 |
| 基因名称 | XDH |
| Gene ID (NCBI) | 7498 |
| RRID | AB_10860905 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P47989 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XDH, also named as XDHA, XO and XD, belongs to the xanthine dehydrogenase family. It catalyzes the last two steps of purine catabolism in man, formation of the end product uric acid from hypoxanthine and xanthine. The mammalian enzyme is synthesized as a dehydrogenase (XDH), which uses NAD as the electron acceptor, but it can be converted into an oxidase (XO) both in vivo and in vitro.This gene encodes a protein of 150 kDa and the 150 kDa polypeptide can be processed into a fragment of 130 kDa and further to 85 kDa by spontaneous proteolytic cleavage(PMID:9989587). In addition, the full length polypeptide can be also partially processed into a 87 kDa and 59 kDa fragments(PMID:21528298). It also can exsit as a homodimer(PMID:9989587). This antibody is specific to XDH.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for XDH antibody 55156-1-AP | Download protocol |
| IHC protocol for XDH antibody 55156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Int J Mol Sci Multiple-Purpose Connectivity Map Analysis Reveals the Benefits of Esculetin to Hyperuricemia and Renal Fibrosis. | ||
Free Radic Biol Med PPARα agonist WY-14,643 enhances ethanol metabolism in mice: role of catalase. | ||
Nutrients Chinese Sumac (Rhus chinensis Mill.) Fruits Prevent Hyperuricemia and Uric Acid Nephropathy in Mice Fed a High-Purine Yeast Diet | ||
Biochem Pharmacol PPARα agonist WY-14,643 induces adipose atrophy and fails to blunt chronic ethanol-induced hepatic fat accumulation in mice lacking adipose FGFR1. | ||
Sci Rep Iron regulatory protein deficiency compromises mitochondrial function in murine embryonic fibroblasts. | ||
Poult Sci Effects of Smilax China L. extracts on Hyperuricemia chicken model via inhibiting xanthine oxidase activity |
