验证数据展示
经过测试的应用
| Positive WB detected in | mouse liver tissue, rat liver tissue |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
55156-1-AP targets XDH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat, chicken, zebrafish, bovine |
| 免疫原 |
Peptide 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | xanthine dehydrogenase |
| 别名 | xanthine dehydrogenase, Xanthine Oxidase, Xanthine oxidoreductase, XD, XDHA |
| 计算分子量 | 146 kDa |
| 观测分子量 | 147-150 kDa |
| GenBank蛋白编号 | NM_000379 |
| 基因名称 | XDH |
| Gene ID (NCBI) | 7498 |
| RRID | AB_10860905 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P47989 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
XDH, also named as XDHA, XO and XD, belongs to the xanthine dehydrogenase family. It catalyzes the last two steps of purine catabolism in man, formation of the end product uric acid from hypoxanthine and xanthine. The mammalian enzyme is synthesized as a dehydrogenase (XDH), which uses NAD as the electron acceptor, but it can be converted into an oxidase (XO) both in vivo and in vitro.This gene encodes a protein of 150 kDa and the 150 kDa polypeptide can be processed into a fragment of 130 kDa and further to 85 kDa by spontaneous proteolytic cleavage(PMID:9989587). In addition, the full length polypeptide can be also partially processed into a 87 kDa and 59 kDa fragments(PMID:21528298). It also can exsit as a homodimer(PMID:9989587).
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for XDH antibody 55156-1-AP | Download protocol |
| WB protocol for XDH antibody 55156-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Psychological stress-induced ALKBH5 deficiency promotes tumour innervation and pancreatic cancer via extracellular vesicle transfer of RNA | ||
Nat Commun Targeting spermine metabolism to overcome immunotherapy resistance in pancreatic cancer | ||
Cell Discov DEPDC5 protects CD8+ T cells from ferroptosis by limiting mTORC1-mediated purine catabolism | ||
Adv Sci (Weinh) Metabolomic and Cellular Mechanisms of Drug-Induced Ototoxicity and Nephrotoxicity: Therapeutic Implications of Uric Acid Modulation | ||
Cancer Lett Hinokitiol preferentially suppresses metastatic lung adenocarcinoma via TMDD1-mediated ferroptosis induction and iron-sulfur cluster inhibition. |
