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验证数据展示

  • WB analysis using 33118-1-AP (same clone as 33118-1-PBS)

    WB analysis using 33118-1-AP (same clone as 33118-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33118-1-AP (XAGE2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33118-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 33118-1-AP (same clone as 33118-1-PBS)

    IF Staining of HeLa using 33118-1-AP (same clone as 33118-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using XAGE2 antibody (33118-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 33118-1-PBS in a different storage buffer formulation.

XAGE2 Polyclonal antibody, PBS Only

XAGE2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 33118-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

Cancer/testis antigen 12.2, CT12.2, G antigen family D member 3, GAGED3, X antigen family member 2

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33118-1-PBS targets XAGE2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag35814

Product name: Recombinant human XAGE2 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 17-111 aa of NM_130777

Sequence: LQPPELIGAMLEPTDEEPKEEKPPTKSRNPTPDQKREDDQGAAEIQVPDLEADLQELCQTKTGDGCEGGTDVKGKILPKAEHFKMPEAGEGKSQV

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 X antigen family, member 2
别名 Cancer/testis antigen 12.2, CT12.2, G antigen family D member 3, GAGED3, X antigen family member 2
计算分子量12kDa
观测分子量20 kDa
GenBank蛋白编号NM_130777
基因名称 XAGE2
Gene ID (NCBI) 9502
RRIDAB_3742862
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96GT9
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

XAGE2 (member 2 of the X antigen family), also known as CT12.2, GAGED3, XAGE-2, and XAGE2B, is a cancer-testis (CT) antigen that belongs to the GAGE family. This protein is strongly expressed in normal testes and is abnormally expressed in various tumors, including Ewing's sarcoma, rhabdomyosarcoma, breast cancer, and germ cell tumors. The XAGE2 protein shares sequence similarity with other GAGE/PAGE proteins, and its expression pattern and sequence similarity make it a member of the CT antigen family.

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