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验证数据展示

  • WB analysis using 15299-1-AP (same clone as 15299-1-PBS)

    WB analysis using 15299-1-AP (same clone as 15299-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15299-1-AP (WWOX antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

    IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WWOX antibody included
  • IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

    IHC staining of human liver cancer using 15299-1-AP (same clone as 15299-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15299-1-AP (WWOX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with WWOX antibody included
  • IP experiment of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

    IP experiment of mouse skeletal muscle using 15299-1-AP (same clone as 15299-1-PBS)

    IP result of anti-WWOX (IP:15299-1-AP, 4ug; Detection:15299-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 15299-1-AP (same clone as 15299-1-PBS)

    WB analysis of MCF-7 using 15299-1-AP (same clone as 15299-1-PBS)

    WB result of WWOX antibody (15299-1-AP; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-WWOX transfected MCF-7 cells. This data was developed using the same antibody clone with 15299-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

WWOX Polyclonal antibody, PBS Only

WWOX Polyclonal Antibody for WB, IHC, IP, Indirect ELISA
Cat No. 15299-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy WWOX Ready-To-Use IHC Kit. WWOX primary antibody included.

产品信息

15299-1-PBS targets WWOX in WB, IHC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag7555

Product name: Recombinant human WWOX protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-234 aa of BC003184

Sequence: MAALRYAGLDDTDSEDELPPGWEERTTKDGWVYYANHTEEKTQWEHPKTGKRKRVAGDLPYGWEQETDENGQVFFVDHINKRTTYLDPRLAFTVDDNPTKPTTRQRYDGSTTAMEILQGRDFTGKVVVVTGANSGIGFETAKSFALHGAHVILACRNMARASEAVSRILEEWQQGAATTVYCAAVPELEGLGGMYFNNCCRCMPSPEAQSEETARTLWALSERLIQERLGSQSG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WW domain containing oxidoreductase
别名
计算分子量 47 kDa
观测分子量 46 kDa
GenBank蛋白编号BC003184
基因名称 WWOX
Gene ID (NCBI) 51741
RRIDAB_2216389
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9NZC7
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WWOX(WW domain-containing oxidoreductase) is also named as FOR, WOX1 and belongs to the short-chain dehydrogenases/reductases (SDR) family. The gene encodes a 46 kDa protein that contains two WW domains and a short-chain dehydrogenase/reductase domain and the protein is lost or reduced in the majority of many cancer types including breast, prostate, esophageal, lung, stomach, and pancreatic carcinomas and in a large fraction of other cancer types(PMID:17360458). WWOX behaves as a potential tumor-suppressor gene(PMID:15064722). It has 7 isoforms produced by alternative splicing.

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