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验证数据展示

  • WB analysis using 81987-4-RR

    WB analysis using 81987-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81987-4-RR (WTAP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of Jurkat using 81987-4-RR

    IF Staining of Jurkat using 81987-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using WTAP antibody (81987-4-RR, Clone: 250657D4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

  • Affinity and Kinetic Characterization of 81987-4-RR

    Affinity and Kinetic Characterization of 81987-4-RR

    Biolayer interferometry (BLl) kinetic assays of 81987-4-RR against Human WTAP were performed. The affinity constant is 5.11 nM.

WTAP Recombinant antibody

WTAP Uni-rAbTM Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 81987-4-RR

产品说明书

CloneNo. 250657D4

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IF/ICC, ELISA

别名

Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2, Pre-mRNA-splicing regulator WTAP

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inJurkat cells
Planning an IHC experiment? We recommend our IHCeasy WTAP Ready-To-Use IHC Kit. WTAP primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81987-4-RR targets WTAP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse
免疫原 WTAP fusion protein Ag0259 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Wilms tumor 1 associated protein
别名 Female-lethal(2)D homolog (hFL(2)D ), FL2D, KIAA0105, Mum2, Pre-mRNA-splicing regulator WTAP
计算分子量 45 kDa
观测分子量 50 kDa
GenBank蛋白编号BC000383
基因名称 WTAP
Gene ID (NCBI) 9589
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15007
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WTAP, also named as KIAA0105 and hFL(2)D, is previously identified as a protein associated with Wilms' tumor-1 (WT-1) protein that is essential for the development of the genitourinary system. It is a Pre-mRNA-splicing regulator protein. It is a regulatory subunit of the WMM N6-methyltransferase complex which plays a role in the efficiency of mRNA splicing and processing and mRNA stability. It is required for accumulation of METTL3 and METTL14 to nuclear speckle. MW of WTAP is 45-50 kDa due to phosphorylation.

实验方案

Product Specific Protocols
WB protocol for WTAP antibody 81987-4-RRDownload protocol
IF protocol for WTAP antibody 81987-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols