• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

WNT8A Polyclonal antibody

WNT8A Polyclonal Antibody for IF/ICC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF/ICC, WB, ELISA

Conjugate

Unconjugated

Cat No : 30518-1-AP

Print datasheet

Synonyms

Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D

验证数据展示

  • WB analysis using 30518-1-AP

    WB analysis using 30518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30518-1-AP (WNT8A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • IF Staining of HCT 116 using 30518-1-AP

    IF Staining of HCT 116 using 30518-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using WNT8A antibody (30518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inCaco-2 cells, HT-29 cells, SW480 cells, rat heart tissue
Positive IF detected inHCT 116 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30518-1-AP targets WNT8A in WB, IF, ELISA applications and shows reactivity with Human, rat samples.

Tested Applications IF/ICC, WB, ELISA
Tested Reactivity Human, rat
Immunogen WNT8A fusion protein Ag29510 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name wingless-type MMTV integration site family, member 8A
Synonyms Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 39-45 kDa
GenBank Accession NumberNM_001300938
Gene Symbol WNT8A
Gene ID (NCBI) 7478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1J5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

实验方案

Product Specific Protocols
WB protocol for WNT8A antibody 30518-1-APDownload protocol
IF protocol for WNT8A antibody 30518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols