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验证数据展示

  • IF Staining of MCF-7 using CL594-66656

    IF Staining of MCF-7 using CL594-66656

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite®594 WNT2 antibody (CL594-66656, Clone: 3H7B10 ) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated WNT2 Monoclonal antibody

WNT2 Monoclonal Antibody for IF/ICC
Cat No. CL594-66656

产品说明书

CloneNo. 3H7B10

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC

别名

3H7B10, Int 1 like protein 1, Int 1 related protein, INT1L1, Int-1-like protein 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 66656-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66656 targets WNT2 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC Application Description
经测试反应性 human, mouse, rat
免疫原 WNT2 fusion protein Ag25185 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 wingless-type MMTV integration site family member 2
别名 3H7B10, Int 1 like protein 1, Int 1 related protein, INT1L1, Int-1-like protein 1
计算分子量 40 kDa
观测分子量 36 kDa
GenBank蛋白编号BC029854
基因名称 WNT2
Gene ID (NCBI) 7472
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDP09544
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Wnt2 is a secreted glycoprotein that is one of the canonical Wnt ligands. The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. Wnt2 is known to directly regulate β-catenin activity, the key player in proliferation and inflammation. Recently, studies have demonstrated that Wnt2 is overexpressed in various human cancers, including esophageal, gastric, colorectal, breast, lung, cervical, and malignant glioma.

实验方案

Product Specific Protocols
IF protocol for CL594 WNT2 antibody CL594-66656Download protocol
Standard Protocols
Click here to view our Standard Protocols