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WISP1 Polyclonal antibody

WISP1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 18166-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA and More (1)

CCN family member 4, CCN4, WISP 1, WISP1, WISP-1, WISP1c, WISP1i, WISP1tc, Wnt 1 induced secreted protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, HEK-293 cells, mouse testis tissue, mouse liver tissue
Positive IHC detected inhuman stomach cancer tissue, human small intestine tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

18166-1-AP targets WISP1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB, IF, IHC
经测试反应性 human, mouse
文献引用反应性human
免疫原 WISP1 fusion protein Ag12657 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WNT1 inducible signaling pathway protein 1
别名 CCN family member 4, CCN4, WISP 1, WISP1, WISP-1, WISP1c, WISP1i, WISP1tc, Wnt 1 induced secreted protein
计算分子量 367 aa, 40 kDa
观测分子量 40-45 kDa
GenBank蛋白编号BC074841
基因名称 WISP1
Gene ID (NCBI) 8840
RRIDAB_10805764
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDO95388
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WISP1( Wnt-induced secreted protein 1), also known as CCN4, is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consists of six, modular structured, secreted proteins ( Cyr61, CTGF, Nov, WISP1, WISP2, WISP3 ). WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production. 18166-1-AP antibody detects the 40-45 kDa protein in SDS-PAGE.

实验方案

Product Specific Protocols
WB protocol for WISP1 antibody 18166-1-APDownload protocol
IHC protocol for WISP1 antibody 18166-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Am J Respir Crit Care Med

Critical Genomic Networks and Vasoreactive Variants in Idiopathic Pulmonary Arterial Hypertension.

Authors - Anna R Hemnes
humanWB,IF

Exp Mol Med

WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling.

Authors - Chao Sun
  • KD Validated
humanWB

Food Chem Toxicol

Quinocetone induces mitochondrial apoptosis in HepG2 cells through ROS-dependent promotion of VDAC1 oligomerization and suppression of Wnt1/β-catenin signaling pathway.

Authors - Xiayun Yang
humanWB, IHC

Am J Transl Res

High expression of WISP-1 correlates with poor prognosis in pancreatic ductal adenocarcinoma.

Authors - Jian-Yu Yang

Int J Oncol

Identification of WISP1 as a novel oncogene in glioblastoma.

Authors - Di Jing
humanWB

Oncol Lett

MMP12 is a potential therapeutic target for Adamantinomatous craniopharyngioma: Conclusions from bioinformatics analysis and in vitro experiments.

Authors - Minde Li
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