验证数据展示
经过测试的应用
| Positive WB detected in | COLO 320 cells, HEK-293 cells, mouse testis tissue, mouse liver tissue |
| Positive IHC detected in | human stomach cancer tissue, human small intestine tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
18166-1-AP targets WISP-1/CCN4 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag12657 Product name: Recombinant human WISP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 132-367 aa of BC074841 Sequence: NGQSFQPNCKYNCTCIDGAVGCTPLCLRVRPPRLWCPHPRRVSIPGHCCEQWVCEDDAKRPRKTAPRDTGAFDAVGEVEAWHRNCIAYTSPWSPCSTSCGLGVSTRISNVNAQCWPEQESRLCNLRPCDVDIHTLIKAGKKCLAVYQPEASMNFTLAGCISTRSYQPKYCGVCMDNRCCIPYKSKTIDVSFQCPDGLGFSRQVLWINACFCNLSCRNPNDIFADLESYPDFSEIAN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WNT1 inducible signaling pathway protein 1 |
| 别名 | WISP1, CCN4, WISP-1, Wnt-1-induced secreted protein, WNT1-inducible-signaling pathway protein 1 |
| 计算分子量 | 367 aa, 40 kDa |
| 观测分子量 | 40-45 kDa |
| GenBank蛋白编号 | BC074841 |
| 基因名称 | WISP1 |
| Gene ID (NCBI) | 8840 |
| RRID | AB_10805764 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | O95388 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WISP1( Wnt-induced secreted protein 1), also known as CCN4, is a secreted, matricellular protein allocated to the CCN protein family. The CCN protein family consists of six, modular structured, secreted proteins ( Cyr61, CTGF, Nov, WISP1, WISP2, WISP3 ). WISP1 may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. WISP1 is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. Its expression is associated with proliferation, cytoprotection, as well as extracellular matrix production. 18166-1-AP antibody detects the 40-45 kDa protein in SDS-PAGE.
实验方案
| Product Specific Protocols | |
|---|---|
| IHC protocol for WISP-1/CCN4 antibody 18166-1-AP | Download protocol |
| WB protocol for WISP-1/CCN4 antibody 18166-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Am J Respir Crit Care Med Critical Genomic Networks and Vasoreactive Variants in Idiopathic Pulmonary Arterial Hypertension. | ||
Exp Mol Med WISP-1 induced by mechanical stress contributes to fibrosis and hypertrophy of the ligamentum flavum through Hedgehog-Gli1 signaling.
| ||
Food Chem Toxicol Quinocetone induces mitochondrial apoptosis in HepG2 cells through ROS-dependent promotion of VDAC1 oligomerization and suppression of Wnt1/β-catenin signaling pathway. | ||
Am J Transl Res High expression of WISP-1 correlates with poor prognosis in pancreatic ductal adenocarcinoma. | ||
Oncol Lett MMP12 is a potential therapeutic target for Adamantinomatous craniopharyngioma: Conclusions from bioinformatics analysis and in vitro experiments. |
