WIPI2 Polyclonal antibody

WIPI2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 28820-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

Human, mouse and More (2)

应用

WB, IHC, IF/ICC, ELISA and More (1)

Atg21, CGI 50, DKFZp434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WIPI 2, WIPI2, WIPI49 like protein 2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inHeLa cells, RAW 264.7 cells
Positive IHC detected inhuman heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28820-1-AP targets WIPI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB, IF
经测试反应性 Human, mouse
文献引用反应性human, mouse, rat
免疫原 WIPI2 fusion protein Ag30505 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain, phosphoinositide interacting 2
别名 Atg21, CGI 50, DKFZp434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WIPI 2, WIPI2, WIPI49 like protein 2
计算分子量 49 kDa
观测分子量 49 kDa
GenBank蛋白编号BC004116
基因名称 WIPI2
Gene ID (NCBI) 26100
RRIDAB_2881218
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9Y4P8
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WIPI2, the mammalian homologue of the yeast ATG18 gene, plays a key role in autophagy. It binds the omegasome, a phosphatidylinositol 3-phosphate (PI3P) rich domain of the endoplasmic reticulum from which the mature autophagosome develops. Western blotting detected a 49 kDa WIPI2 band.(PMID: 30968111, PMID: 30403914)

实验方案

Product Specific Protocols
WB protocol for WIPI2 antibody 28820-1-APDownload protocol
IHC protocol for WIPI2 antibody 28820-1-APDownload protocol
IF protocol for WIPI2 antibody 28820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

COPI Vesicle Disruption Inhibits Mineralization via mTORC1-Mediated Autophagy

Authors - Jiaming Nie
mouseWB

Autophagy

TBC1D4 antagonizes RAB2A-mediated autophagic and endocytic pathways

Authors - Rui Tian
ratIF

Adv Sci (Weinh)

SEC31a-ATG9a Interaction Mediates the Recruitment of COPII Vesicles for Autophagosome Formation

Authors - Jiaming Nie
WB

Cell Death Differ

Vps34 sustains Treg cell survival and function via regulating intracellular redox homeostasis

Authors - Peiran Feng
IF

Autophagy

Regulation of N-Degron Recognin-Mediated Autophagy by the sars-cov-2 plpro ubiquitin deconjugase

Authors - Carlos Ayala Torres
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