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WIPI2 Polyclonal antibody

WIPI2 Polyclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

Cat No : 28820-1-AP

Print datasheet

Synonyms

Atg21, CGI 50, DKFZp434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WIPI 2, WIPI2, WIPI49 like protein 2

验证数据展示

  • IHC staining of human heart using 28820-1-AP

    IHC staining of human heart using 28820-1-AP

    Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 28820-1-AP (WIPI2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 28820-1-AP

    IF Staining of HeLa using 28820-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WIPI2 antibody (28820-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

  • WB analysis using 28820-1-AP

    WB analysis using 28820-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 28820-1-AP (WIPI2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHeLa cells, RAW 264.7 cells
Positive IHC detected inhuman heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

28820-1-AP targets WIPI2 in WB, IF, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Cited ApplicationsIF
Tested Reactivity Human, mouse
Cited Reactivityhuman
Immunogen WIPI2 fusion protein Ag30505 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain, phosphoinositide interacting 2
Synonyms Atg21, CGI 50, DKFZp434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WIPI 2, WIPI2, WIPI49 like protein 2
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC004116
Gene Symbol WIPI2
Gene ID (NCBI) 26100
RRIDAB_2881218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y4P8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WIPI2, the mammalian homologue of the yeast ATG18 gene, plays a key role in autophagy. It binds the omegasome, a phosphatidylinositol 3-phosphate (PI3P) rich domain of the endoplasmic reticulum from which the mature autophagosome develops. Western blotting detected a 49 kDa WIPI2 band.(PMID: 30968111, PMID: 30403914)

实验方案

Product Specific Protocols
WB protocol for WIPI2 antibody 28820-1-APDownload protocol
IHC protocol for WIPI2 antibody 28820-1-APDownload protocol
IF protocol for WIPI2 antibody 28820-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

COPI Vesicle Disruption Inhibits Mineralization via mTORC1-Mediated Autophagy

Authors - Jiaming Nie
View Article