验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, RAW 264.7 cells |
Positive IHC detected in | human heart tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:2000-1:16000 |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
WB | See 2 publications below |
IF | See 3 publications below |
产品信息
28820-1-AP targets WIPI2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.
经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
文献引用应用 | WB, IF |
经测试反应性 | Human, mouse |
文献引用反应性 | human, mouse, rat |
免疫原 | WIPI2 fusion protein Ag30505 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Polyclonal |
产品类型 | Antibody |
全称 | WD repeat domain, phosphoinositide interacting 2 |
别名 | Atg21, CGI 50, DKFZp434J154, DKFZp686P02188, FLJ12979, FLJ14217, FLJ42984, WIPI 2, WIPI2, WIPI49 like protein 2 |
计算分子量 | 49 kDa |
观测分子量 | 49 kDa |
GenBank蛋白编号 | BC004116 |
基因名称 | WIPI2 |
Gene ID (NCBI) | 26100 |
RRID | AB_2881218 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Antigen affinity purification |
UNIPROT ID | Q9Y4P8 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WIPI2, the mammalian homologue of the yeast ATG18 gene, plays a key role in autophagy. It binds the omegasome, a phosphatidylinositol 3-phosphate (PI3P) rich domain of the endoplasmic reticulum from which the mature autophagosome develops. Western blotting detected a 49 kDa WIPI2 band.(PMID: 30968111, PMID: 30403914)
实验方案
Product Specific Protocols | |
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WB protocol for WIPI2 antibody 28820-1-AP | Download protocol |
IHC protocol for WIPI2 antibody 28820-1-AP | Download protocol |
IF protocol for WIPI2 antibody 28820-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Int J Mol Sci COPI Vesicle Disruption Inhibits Mineralization via mTORC1-Mediated Autophagy | ||
Adv Sci (Weinh) SEC31a-ATG9a Interaction Mediates the Recruitment of COPII Vesicles for Autophagosome Formation | ||
Cell Death Differ Vps34 sustains Treg cell survival and function via regulating intracellular redox homeostasis | ||
Autophagy Regulation of N-Degron Recognin-Mediated Autophagy by the sars-cov-2 plpro ubiquitin deconjugase |