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验证数据展示

  • WB analysis of A549 using 83116-2-RR (same clone as 83116-2-PBS)

    WB analysis of A549 using 83116-2-RR (same clone as 83116-2-PBS)

    WB result of NSD3 antibody (83116-2-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NSD3 transfected A549 cells. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83116-2-RR (same clone as 83116-2-PBS)

    FC experiment of HeLa using 83116-2-RR (same clone as 83116-2-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug NSD3 Recombinant antibody (83116-2-RR, Clone:230499C2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • WB analysis using 83116-2-RR (same clone as 83116-2-PBS)

    WB analysis using 83116-2-RR (same clone as 83116-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83116-2-RR (WHSC1L1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4

    Sandwich ELISA standard curve of MP00189-4, Human NSD3 Recombinant Matched Antibody Pair - PBS only. 83116-2-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag1894. 83116-5-PBS was HRP conjugated as the detection antibody. Range: 19.5-625 pg/mL.

  • Sample test of MP00189-4

    Sample test of MP00189-4

    The mean NSD3 concentration was determined to be 542.5 pg/mL in A549 cell extract based on a 1.0 mg/mL extract load, 565.1 pg/mL in HEK-293 cell extract based on a 1.2 mg/mL extract load and 529.6 pg/mL in HeLa cell extract based on a 1.5 mg/mL extract load.

  • IHC staining of human lung squamous cell carcinoma using 83116-2-RR (same clone as 83116-2-PBS)

    IHC staining of human lung squamous cell carcinoma using 83116-2-RR (same clone as 83116-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung squamous cell carcinoma tissue slide using 83116-2-RR (NSD3 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 83116-2-RR (same clone as 83116-2-PBS)

    IF Staining of A549 using 83116-2-RR (same clone as 83116-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using NSD3 antibody (83116-2-RR, Clone: 230499C2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83116-2-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83116-2-RR

    Affinity and Kinetic Characterization of 83116-2-RR

    Biolayer interferometry (BLl) kinetic assays of 83116-2-RR against Human NSD3 were performed. The affinity constant is below 1 pM.

  • Featured Product
  • KD/KO验证

NSD3 Recombinant antibody, PBS Only (Capture)

NSD3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test
Cat No. 83116-2-PBS

产品说明书

CloneNo. 230499C2

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test

别名

WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83116-2-PBS targets NSD3 as part of a matched antibody pair:

MP00189-4: 83116-2-PBS capture and 83116-5-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Sandwich ELISA, Indirect ELISA, Sample test Application Description
经测试反应性 human
免疫原 NSD3 fusion protein Ag1894 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 Wolf-Hirschhorn syndrome candidate 1-like 1
别名 WHSC1L1, DC28, EC:2.1.1.370, Histone-lysine N-methyltransferase NSD3, Nuclear SET domain-containing protein 3
计算分子量 1437 aa, 162 kDa
观测分子量 160-170 kDa, 68-72 kDa
GenBank蛋白编号BC012059
基因名称 WHSC1L1
Gene ID (NCBI) 54904
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ9BZ95
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WHSC1L1, also named as NSD3 and DC28, belongs to the histone-lysine methyltransferase family and SET2 subfamily. It is histone methyltransferase. WHSC1L1 is a potently transforming oncogenes based on the number of altered phenotypes expressed by the cells. It contains a PWWP-domain that is a methyl-lysine recognition motif involved in histone code modification and epigenetic regulation of gene expression. Knockdown of WHSC1L1 in 8p11-12-amplified breast cancer cells resulted in profound loss of growth and survival of these cells. (PMID: 20940404). This antibody recognize the long isoform (160-170 kDa) and short isoform (68-72 kDa).