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验证数据展示

  • WB analysis using 31572-1-AP (same clone as 31572-1-PBS)

    WB analysis using 31572-1-AP (same clone as 31572-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31572-1-AP (WHAMM antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31572-1-PBS in a different storage buffer formulation.

  • IF Staining of A431 using 31572-1-AP (same clone as 31572-1-PBS)

    IF Staining of A431 using 31572-1-AP (same clone as 31572-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using WHAMM antibody (31572-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 31572-1-PBS in a different storage buffer formulation.

WHAMM Polyclonal antibody, PBS Only

WHAMM Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 31572-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA1971, WHDC1

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

31572-1-PBS targets WHAMM in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag36160

Product name: Recombinant human WHAMM protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 660-809 aa of BC167774

Sequence: RALSSSSQAATHQNLGFRAPVKDDQPRPLVCESPAERPRDSLESFSCPGSMDEVLASLRHGRAPLRKVEVPAVRPPHASINEHILAAIRQGVKLKKVHPDLGPNPSSKPTSNRRTSDLERSIKAALQRIKRVSADSEEDSDEQDPGQWDG

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WAS protein homolog associated with actin, golgi membranes and microtubules
别名 KIAA1971, WHDC1
观测分子量90-100 kDa
GenBank蛋白编号BC167774
基因名称 WHAMM
Gene ID (NCBI) 123720
RRIDAB_3670039
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ8TF30
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WHAMM (WASP homolog associated with actin, golgi membranes and microtubules), also known as WHDC1. It is expected to be located in the cytoplasm, which is enriched in brain, lung, heart, colon and kidney. WHAMM is involved in Golgi membrane association, microtubule binding, and actin nucleation as a nucleation-promoting factor, which activates the actin-related protein 2/3 complex (the Arp2/ 3 complex) (PMID: 23160625). It is reported that the nucleation-promoting factor (NPF) WHAMM recruits and activates the Arp2/3 complex for actin assembly at sites of autophagosome formation on the ER (PMID: 26291929). The molecular weight of WHAMM is 90 kDa.

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