验证数据展示
经过测试的应用
| Positive WB detected in | Daudi cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
31572-1-AP targets WHAMM in WB, ELISA applications and shows reactivity with human samples.
| 经测试应用 | WB, ELISA Application Description |
| 经测试反应性 | human |
| 免疫原 | WHAMM fusion protein Ag36160 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WAS protein homolog associated with actin, golgi membranes and microtubules |
| 别名 | WHDC1, KIAA1971 |
| 观测分子量 | 90-100 kDa |
| GenBank蛋白编号 | BC167774 |
| 基因名称 | WHAMM |
| Gene ID (NCBI) | 123720 |
| RRID | AB_3670039 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity Purification |
| UNIPROT ID | Q8TF30 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WHAMM (WASP homolog associated with actin, golgi membranes and microtubules), also known as WHDC1. It is expected to be located in the cytoplasm, which is enriched in brain, lung, heart, colon and kidney. WHAMM is involved in Golgi membrane association, microtubule binding, and actin nucleation as a nucleation-promoting factor, which activates the actin-related protein 2/3 complex (the Arp2/ 3 complex) (PMID: 23160625). It is reported that the nucleation-promoting factor (NPF) WHAMM recruits and activates the Arp2/3 complex for actin assembly at sites of autophagosome formation on the ER (PMID: 26291929). The molecular weight of WHAMM is 90 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WHAMM antibody 31572-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |