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验证数据展示

  • WB analysis using 33483-1-AP (same clone as 33483-1-PBS)

    WB analysis using 33483-1-AP (same clone as 33483-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 33483-1-AP (WDSOF1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 33483-1-PBS in a different storage buffer formulation.

  • IHC staining of human ovary tumor using 33483-1-AP (same clone as 33483-1-PBS)

    IHC staining of human ovary tumor using 33483-1-AP (same clone as 33483-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 33483-1-AP (WDSOF1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 33483-1-PBS in a different storage buffer formulation.

  • IF Staining of PC-3 using 33483-1-AP (same clone as 33483-1-PBS)

    IF Staining of PC-3 using 33483-1-AP (same clone as 33483-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using WDSOF1 antibody (33483-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 33483-1-PBS in a different storage buffer formulation.

WDSOF1 Polyclonal antibody, PBS Only

WDSOF1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 33483-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

DCAF13, DDB1 And CUL4 Associated Factor 13, DDB1- and CUL4-associated factor 13, HSPC064, WD repeat and SOF domain-containing protein 1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

33483-1-PBS targets WDSOF1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag35559

Product name: Recombinant human WDSOF1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 249-597 aa of BC112042

Sequence: TQRNCIRTIQAHEGFVRGICTRFCGTSFFTVGDDKTVKQWKMDGPGYGDEEEPLHTILGKTVYTGIDHHWKEAVFATCGQQVDIWDEQRTNPICSMTWGFDSISSVKFNPIETFLLGSCASDRNIVLYDMRQATPLKKVILDMRTNTICWNPMEAFIFTAANEDYNLYTFDMRALDTPVMVHMDHVSAVLDVDYSPTGKEFVSASFDKSIRIFPVDKSRSREVYHTKRMQHVICVKWTSDSKYIMCGSDEMNIRLWKANASEKLGVLTSREKAAKDYNQKLKEKFQHYPHIKRIARHRHLPKSIYSQIQEQRIMKEARRRKEVNRIKHSKPGSVPLVSEKKKHVVAVVK

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeats and SOF1 domain containing
别名 DCAF13, DDB1 And CUL4 Associated Factor 13, DDB1- and CUL4-associated factor 13, HSPC064, WD repeat and SOF domain-containing protein 1
计算分子量 597 aa, 67 kDa
观测分子量60 kDa
GenBank蛋白编号BC112042
基因名称 WDSOF1
Gene ID (NCBI) 25879
RRIDAB_3742988
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ9NV06
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

WDSOF1 (WD repeat and SOF1 domain-containing 1, also known as DCAF13) functions as a substrate receptor subunit for the CRL4-DDB1 E3 ubiquitin ligase complex. It contains WD40 repeat clusters and is primarily localized in the nucleolus, where it participates in the processing of 40S ribosomal RNA precursors and the regulation of the estrogen receptor. Recent research has revealed its indispensable role in uterine development: its deficiency leads to the downregulation of estrogen target genes, an increase in H3K9me3 levels, failure of embryo implantation, and complete infertility in female mice. Furthermore, WDSOF1 is located at 8q22.3, a genomic region frequently amplified in tumors. It is highly expressed in various cancers, including liver and breast cancer, and is associated with poor prognosis, positioning it as an oncogenic factor and a potential therapeutic target.

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