验证数据展示
经过测试的应用
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | human liver tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
24431-1-AP targets WDR7 in WB, IF, IHC, ELISA applications and shows reactivity with human, rat, mouse samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | IF |
| 经测试反应性 | human, rat, mouse |
| 免疫原 |
CatNo: Ag19072 Product name: Recombinant human WDR7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1145-1490 aa of BC131558 Sequence: LLTRPRSSSQIPEGFGLTSGGSNYSLARHTCKALTFLLLQPPSPKLPPHSTIRRTAIDLIGRGFTVWEPYMDVSAVLMGLLELCADAEKQLANITMGLPLSPAADSARSARHALSLIATARPPAFITTIAKEVHRHTALAANTQSQQNMHTTTLARAKGEILRVIEILIEKMPTDVVDLLVEVMDIIMYCLEGSLVKKKGLQECFPAICRFYMVSYYERNHRIAVGARHGSVALYDIRTGKCQTIHGHKGPITAVAFAPDGRYLATYSNTDSHISFWQMNTSLLGSIGMLNSAPQLRCIKTYQVPPVQPASPGSHNALKLARLIWTSNRNVILMAHDGKEHRFMV 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | WD repeat domain 7 |
| 别名 | KIAA0541, Rabconnectin 3 beta, TRAG, WD repeat containing protein 7, WD repeat domain 7, WDR7 |
| 计算分子量 | 1490 aa, 164 kDa |
| 观测分子量 | 145-150 kDa |
| GenBank蛋白编号 | BC131558 |
| 基因名称 | WDR7 |
| Gene ID (NCBI) | 23335 |
| RRID | AB_2879546 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q9Y4E6 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
WDR7, also named as KIAA0541 and TRAG, belongs to the WD-repeat protein family. WD repeats are areas with the repetitions of approximately 40 amino acids that have ends with trp-asp and gly-his amino acids (GH-WD) which may help to form a multi-protein complex. WDR7 is the gene encoding adjacent of lincRNA-RoR, which has an important role in apoptosis and cell cycle (PMID: 32128092). WDR7 has 2 isoforms with the molecular mass of 160 and 164 kDa. Sometimes lower molecular weight around 150 kDa can also be observed, which may be a modified variant of WDR7.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for WDR7 antibody 24431-1-AP | Download protocol |
| IHC protocol for WDR7 antibody 24431-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
