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验证数据展示

  • WB analysis using 20786-1-AP

    WB analysis using 20786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20786-1-AP (WDR55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20786-1-AP

    IHC staining of human liver cancer using 20786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20786-1-AP (WDR55 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 20786-1-AP

    IP experiment of HeLa using 20786-1-AP

    IP result of anti-WDR55 (IP:20786-1-AP, 4ug; Detection:20786-1-AP 1:1000) with HeLa cells lysate 680 ug.

  • IF Staining of HepG2 using 20786-1-AP

    IF Staining of HepG2 using 20786-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR55 antibody (20786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

WDR55 Polyclonal antibody

WDR55 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 20786-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, IP, ELISA

别名

WD repeat domain 55, WDR55

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20786-1-AP targets WDR55 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag14241

Product name: Recombinant human WDR55 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 162-383 aa of BC002482

Sequence: MDMRQHEEYIADMALDPAKKLLLTASGDGCLGIFNIKRRRFELLSEPQSGDLTSVTLMKWGKKVACGSSEGTIYLFNWNGFGATSDRFALRAESIDCMVPVTESLLCTGSTDGVIRAVNILPNRVVGSVGQHTGEPVEELALSHCGRFLASSGHDQRLKFWDMAQLRAVVVDDYRRRKKKGGPLRALSSKTWSTDDFFAGLREEGEDSMAQEEKEETGDDSD

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 WD repeat domain 55
别名 WD repeat domain 55, WDR55
计算分子量 383 aa, 42 kDa
观测分子量 42-50 kDa
GenBank蛋白编号BC002482
基因名称 WDR55
Gene ID (NCBI) 54853
RRIDAB_2878739
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9H6Y2
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WD repeat domain 55 (WDR55) also named as, FLJ20195, is a 383 amino acid protein, which contains seven WD repeats and belongs to the WD repeat WDR55 family. WDR55 localizes in the nucleus and cytoplasm. WDR55 acts as a modulator of rRNA synthesis and play a central role in organogenesis.

实验方案

Product Specific Protocols
WB protocol for WDR55 antibody 20786-1-APDownload protocol
IHC protocol for WDR55 antibody 20786-1-APDownload protocol
IF protocol for WDR55 antibody 20786-1-APDownload protocol
IP protocol for WDR55 antibody 20786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols