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WDR55 Polyclonal antibody

WDR55 Polyclonal Antibody for WB,IP,IHC,IF/ICC,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB,IP,IHC,IF/ICC,ELISA

Conjugate

Unconjugated

Cat No : 20786-1-AP

Print datasheet

Synonyms

WD repeat domain 55, WDR55

验证数据展示

  • WB analysis using 20786-1-AP

    WB analysis using 20786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 20786-1-AP (WDR55 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver cancer using 20786-1-AP

    IHC staining of human liver cancer using 20786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 20786-1-AP (WDR55 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IP experiment of HeLa using 20786-1-AP

    IP experiment of HeLa using 20786-1-AP

    IP result of anti-WDR55 (IP:20786-1-AP, 4ug; Detection:20786-1-AP 1:1000) with HeLa cells lysate 680 ug.

  • IF Staining of HepG2 using 20786-1-AP

    IF Staining of HepG2 using 20786-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using WDR55 antibody (20786-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

经过测试的应用

Positive WB detected inHeLa cells, Jurkat cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20786-1-AP targets WDR55 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,IHC,IF/ICC,ELISA
Tested Reactivity human
Immunogen WDR55 fusion protein Ag14241 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 55
Synonyms WD repeat domain 55, WDR55
Calculated Molecular Weight 383 aa, 42 kDa
Observed Molecular Weight 42-50 kDa
GenBank Accession NumberBC002482
Gene Symbol WDR55
Gene ID (NCBI) 54853
RRIDAB_2878739
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H6Y2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WD repeat domain 55 (WDR55) also named as, FLJ20195, is a 383 amino acid protein, which contains seven WD repeats and belongs to the WD repeat WDR55 family. WDR55 localizes in the nucleus and cytoplasm. WDR55 acts as a modulator of rRNA synthesis and play a central role in organogenesis.

实验方案

Product Specific Protocols
WB protocol for WDR55 antibody 20786-1-APDownload protocol
IHC protocol for WDR55 antibody 20786-1-APDownload protocol
IF protocol for WDR55 antibody 20786-1-APDownload protocol
IP protocol for WDR55 antibody 20786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols