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WDR35 Polyclonal antibody

WDR35 Polyclonal Antibody for IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, Canine

Applications

IF/ICC, ELISA

Conjugate

Unconjugated

Cat No : 26701-1-AP

Print datasheet

Synonyms

IFT121, KIAA1336, WD repeat domain 35, WDR35

验证数据展示

  • IF Staining of MDCK using 26701-1-AP

    IF Staining of MDCK using 26701-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MDCK cells using WDR35 antibody (26701-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

  • IF Staining of hTERT-RPE1 using 26701-1-AP

    IF Staining of hTERT-RPE1 using 26701-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed hTERT-RPE1 cells using WDR35 antibody (26701-1-AP) at dilution of 1:400 and CoraLite®594-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CoraLite® Plus 488 ARL13B antibody (CL488-17711, green).

经过测试的应用

Positive IF detected inMDCK cells, hTERT-RPE1 cells

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

26701-1-AP targets WDR35 in IF, ELISA applications and shows reactivity with human, Canine samples.

Tested Applications IF/ICC, ELISA
Tested Reactivity human, Canine
Immunogen WDR35 fusion protein Ag24965 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name WD repeat domain 35
Synonyms IFT121, KIAA1336, WD repeat domain 35, WDR35
Calculated Molecular Weight 1170 aa, 132 kDa
GenBank Accession NumberBC036659
Gene Symbol WDR35
Gene ID (NCBI) 57539
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2L0
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WDR35, a component of the IFT complex A (IFT-A), is required for retrograde ciliary transport and entry into cilia of G protein-coupled receptors (GPCRs). It is involved in ciliogenesis and ciliary protein trafficking (PMID:21473986, PMID:28400947, PMID:29220510). It may promote CASP3 activation and TNF-stimulated apoptosis (PMID: 20193664).

实验方案

Product Specific Protocols
IF protocol for WDR35 antibody 26701-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols